Proteoglycans in primate arteries. II. Synthesis and secretion of glycosaminoglycans by arterial smooth muscle cells in culture
Proteoglycans in primate arteries. II. Synthesis and secretion of glycosaminoglycans by arterial smooth muscle cells in culture
复制标题
灵长类动物动脉中的蛋白多糖。
作者：
T. Wight;R. Ross
灵长类动物动脉平滑肌糖胺聚糖的合成和分泌已在细胞培养中进行了研究。将二倍体灵长类动物动脉平滑肌细胞的大量培养物用[35 S]硫酸盐和[3 H]乙酸盐双标记或用[3 H]葡糖胺单标记24 h，并从培养基中提取和分离糖胺聚糖。掺入标记的前体糖胺聚糖是最大的平滑肌细胞生长在培养的稳定期和减少，但在对数生长过程中没有消除。糖胺聚糖的合成和分泌到培养基中，其特征在于由不同的敏感性糖胺聚糖降解酶和醋酸纤维素电泳。两种测定方法都表明培养的灵长类动物动脉平滑肌细胞主要合成硫酸皮肤素（占总量的60%-80%）、硫酸软骨素A和/或C（占总量的10%-20%）和很少或不合成透明质酸（占总量的0%-5%）。这种糖胺聚糖形成的模式与在相同条件下培养的islobulin皮肤成纤维细胞所表现出的模式显著不同。真皮成纤维细胞主要合成和分泌透明质酸（占总量的50%-60%），以及少量的硫酸皮肤素（占总量的10%-20%）和硫酸软骨素A和/或C（占总量的10%-20%）。这些结果表明，这两个结缔组织生产细胞在体外的蛋白多糖代谢之间存在差异，并建议在体外观察到的灵长类动物动脉平滑肌细胞的糖胺聚糖合成的模式可能是这种细胞类型的特征，而不是一般的细胞培养条件的反应。
Glycosaminoglycan synthesis and secretion by primate arterial smooth muscle have been examined in cell culture. Mass cultures of diploid primate arterial smooth muscle cells were either double labeled with [35S]sulfate and [3H]acetate or single labeled with [3H]glucosamine for 24 h and glycosaminoglycans were extracted and isolated from the culture medium. Incorporation of labeled precursors into glycosaminoglycan was maximal during stationary phase of smooth muscle cell growth in culture and reduced, but not eliminated during logarithmic growth. The glycosaminoglycans synthesized and secreted into the culture medium were characterized by differential susceptibility to glycosaminoglycan-degradative enzymes and by cellulose acetate electrophoresis. Both assay procedures indicate that cultured primate arterial smooth muscle cells synthesize principally dermatan sulfate (60%-80% of total), chondroitin sulfate A and/or C (10%-20%of total) and little or no hyaluronic acid (0%-5% of total). This pattern of glycosaminoglycan formation differed significantly from that exhibited by isologous skin fibroblasts cultured under identical conditions. Dermal fibroblasts synthesize and secrete primarily hyaluronic acid (50%-60% of total) with lesser amounts of dermatan sulfate (10%-20% of total) and chondroitin sulfate A and/or C (10%-20% of total). These results indicate that differences exist in proteoglycan metabolism between these two connective tissue-producing cells in vitro, and suggest that the observed pattern of in vitro glycosaminoglycan synthesis by primate arterial smooth muscle cells may be characteristic for this cell type and not a general response to conditions of cell culture.