Riverine distribution of mussel environmental DNA reflects a balance among density, transport, and removal processes
Riverine distribution of mussel environmental
DNA
reflects a balance among density, transport, and removal processes
复制标题
贻贝环境 DNA 的河流分布反映了密度、运输和清除过程之间的平衡
作者：
Arial J. Shogren;J. Tank;S. Egan;D. Bolster;T. Riis
环境DNA（eDNA）水采样是一种新兴工具，可以在无需直接观察的情况下记录物种的存在，从而比水生生态系统中的传统采样方法更早地检测和更快地响应。然而，目前对eDNA如何在溪流和河流中运输的理解仍然不精确，不确定eDNA的独特运输特性如何影响阳性检测的解释。为了测试流动沃茨中eDNA传感的实用性，我们比较了定量eDNA分析斑马贻贝密度调查在丹麦的河流。虽然流水使eDNA的生产，运输和去除之间的关系复杂化，我们发现eDNA浓度，斑马贻贝和生物物理参数之间存在微弱但正相关的关系。例如，虽然斑马贻贝密度只有适度的预测eDNA浓度，eDNA是最强烈的影响营养浓度和水流速度。这些结果可用于通知未来的采样策略，水文变量可以更好地约束eDNA的命运。我们还对净eDNA运输、保留和降解的估计进行了建模，以估计这些过程对从水柱中去除eDNA的相对重要性。在我们的研究系统中，身体保留占c。与单独降解相比，去除率为70%，使其成为评估下游eDNA运输时需要考虑的重要过程。
Sampling water for environmental DNA (eDNA) is an emerging tool for documenting species presence without direct observation, allowing for earlier detection and faster response than conventional sampling methods in aquatic ecosystems. However, current understanding of how eDNA is transported in streams and rivers remains imprecise, with uncertainty of how the unique transport properties of eDNA may influence the interpretation of a positive detection. To test the utility of eDNA sensing in flowing waters, we compared quantitative eDNA analyses to zebra mussel density surveys in a Danish river. Although flowing water complicates the relationships between eDNA production, transport, and removal, we found weak but positive relationships between eDNA concentration, zebra mussels, and biophysical parameters. For example, while zebra mussel densities were only moderately predicted by eDNA concentrations, eDNA was most strongly influenced by nutrient concentrations and water velocity. These results may be used to inform future sampling strategies, where hydrological variables could better constrain eDNA fate. We also modelled estimates for net eDNA transport, retention, and degradation to estimate the relative importance of these processes for removing eDNA from the water column. In our study system, physical retention accounted for c. 70% of removal when compared to degradation alone, making it an important process to consider when assessing downstream eDNA transport.
影响因子：
4.4
作者：
N. B. Svenningsen;Ines M. Heisterkamp;Maria Sigby-Clausen;L. H. Larsen;L. Nielsen;P. Stief;A. Schramm
通讯作者：
N. B. Svenningsen;Ines M. Heisterkamp;Maria Sigby-Clausen;L. H. Larsen;L. Nielsen;P. Stief;A. Schramm