Empirical establishment of oligonucleotide probe design criteria
Empirical establishment of oligonucleotide probe design criteria
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DOI：
10.1128/aem.71.7.3753-3760.2005
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发表时间：
2005-07-01
影响因子：
4.4
通讯作者：
Zhou, JZ
中科院分区：
文献类型：
--
作者：
He, ZL;Wu, LY;Zhou, JZ
基因特异性和组特异性寡核苷酸探针的设计标准通过包含基于四个基因的完全匹配（PM）和错配探针（50-mer和70-mer）的寡核苷酸阵列实验建立。测试了探针-靶标同一性、连续拉伸、错配位置和杂交自由能对特异性的影响。在15个碱基的探针-靶同一性下观察到很少的杂交）与非拉伸探针相比贡献了额外的9%的PM信号强度（
Criteria for the design of gene-specific and group-specific oligonucleotide probes were established experimentally via an oligonucleotide array that contained perfect match (PM) and mismatch probes (50-mers and 70-mers) based upon four genes. The effects of probe-target identity, continuous stretch, mismatch position, and hybridization free energy on specificity were tested. Little hybridization was observed at a probe-target identity of 15 bases) contributed an additional 9% of the PM signal intensity compared to a nonstretch probe (