Comparison of L-type amino acid transporter 1 expression and L-[3-18F]-α-methyl tyrosine uptake in outcome of non-small cell lung cancer
Comparison of L-type amino acid transporter 1 expression and L-[3-18F]-α-methyl tyrosine uptake in outcome of non-small cell lung cancer
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DOI：
10.1016/j.nucmedbio.2010.06.004
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发表时间：
2010-11-01
影响因子：
3.1
通讯作者：
Endo, Keigo
中科院分区：
文献类型：
--
作者：
Kaira, Kyoichi;Oriuchi, Noboru;Endo, Keigo
目的：L氨基酸转运体1(LAT1)与非小细胞肺癌(NSCLC)的肿瘤生长和不良预后有关。L-[3-F-18]-α-甲基酪氨酸(F-18-FAMT)是一种用于正电子发射断层扫描的氨基酸示踪剂，摄取F-18-FAMT是由LAT1介导的。本研究的目的是比较F-18-FAMT在原发肿瘤中的摄取与LAT1在非小细胞肺癌患者中表达的预后意义。所有患者在肿瘤切除前均行F-18-FAMT PET，并对切除的肿瘤进行免疫组织化学染色，比较F-18-FAMT摄取和LAT1的表达。用半定量标准摄取值(SUVmax)评价F-18-FAMT的摄取，并确定SUVmax高的患者与低SUVmax的患者的临界值。结果：F-18-FAMT SUVmax x在原发肿瘤中的最佳分界值为1.6。F-18-FAMT PET的高SUVmax(>；1.6)与男性显著相关，LAT1阳性表达与男性及非腺癌显著相关。在单变量分析中，F-18-FAMT PET中高SUVmax(>；1.6)和LAT1阳性表达是预后不良的显著预测因素。多因素分析证实，LAT1阳性表达是预测NSCLC预后不良的独立显著因素(P=0.035)。结论：LAT1表达是比F-18-FAMT摄取更强的预后因素。(C)2010 Elsevier Inc.保留所有权利。
Objective: L-Type amino acid transporter 1 (LAT1) has associated with tumor growth and poor outcome of patients with non-small cell lung cancer (NSCLC). L-[3-F-18]-alpha-methyl tyrosine (F-18-FAMT) is an amino acid tracer for positron emission tomography (PET) imaging, and F-18-FAMT uptake is mediated by LAT1. The purpose of this study is to compare the prognostic significance of F-18-FAMT uptake in the primary tumors with that of LAT1 expression in patients with NSCLC.Methods: Fifty-nine patients with NSCLC were enrolled in this study. All patients underwent F-18-FAMT PET prior to resection of the tumor, and immunohistochemical staining of the resected tumors were performed to compare the F-18-FAMT uptake and LAT1 expression. Uptake of F-18-FAMT was evaluated using semiquantitative standardized uptake value (SUVmax), and the cutoff value was determined to discriminate patients with high SUVmax from those with low SUVmax. Expression of LAT1 was evaluated by the score of staining intensity through 1 to 4. SUVmax and LAT1 expression were compared according to the clinicopathological variables.Results: The best discriminative cutoff value of F-18-FAMT SUVmax x within the primary tumors was 1.6. The high SUVmax (>1.6) in F-18-FAMT PET was significantly associated with male, and positive LAT1 expression was significantly associated with male and nonadenocarcinoma. In the univariate analysis, high SUVmax (>1.6) in F-18-FAMT PET and positive LAT1 expression were significant predictor of the poor outcome. Multivariate analysis confirmed that positive LAT1 expression was an independent and significant factor for predicting poor prognosis in NSCLC (P=.035).Conclusion: LAT1 expression is a stronger prognostic factor than F-18-FAMT uptake in surgically resected NSCLC. (C) 2010 Elsevier Inc. All rights reserved.