Collective epithelial migration and cell rearrangements drive mammary branching morphogenesis
Collective epithelial migration and cell rearrangements drive mammary branching morphogenesis
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DOI：
10.1016/j.devcel.2008.03.003
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发表时间：
2008-04-01
影响因子：
11.8
通讯作者：
Werb, Zena
中科院分区：
文献类型：
--
作者：
Ewald, Andrew J.;Brenot, Audrey;Werb, Zena
上皮器官是通过一组相互连接的细胞的运动来建立的。我们观察到延长的乳腺导管中的细胞重组成多层上皮，集体迁移，并动态重新排列，所有这些都没有形成领先的细胞延伸。导管的启动需要增殖、RAC和肌球蛋白轻链激酶，而复极化到双层依赖于Rho激酶。我们观察到，分支形态的发生是腔上皮细胞和肌上皮细胞主动运动的结果。腔上皮细胞集体推进，而肌上皮细胞似乎抑制延长的导管。值得注意的是，我们观察到正常上皮和肿瘤性增生性病变的组织结构相似，这表明了上皮生长的共同机制。
Epithelial organs are built through the movement of groups of interconnected cells. We observed cells in elongating mammary ducts reorganize into a multilayered epithelium, migrate collectively, and rearrange dynamically, all without forming leading cellular extensions. Duct initiation required proliferation, Rac, and myosin light-chain kinase, whereas repolarization to a bilayer depended on Rho kinase. We observed that branching morphogenesis results from the active motility of both luminal and myoepithelial cells. Luminal epithelial cells advanced collectively, whereas myoepithelial cells appeared to restrain elongating ducts. Significantly, we observed that normal epithelium and neoplastic hyperplasias are organized similarly, suggesting common mechanisms of epithelial growth.