Overall linkage map of the nonstructural proteins of Aichi virus
Overall linkage map of the nonstructural proteins of Aichi virus
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DOI：
10.1016/j.virusres.2009.10.009
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发表时间：
2010-01-01
期刊：
影响因子：
5
通讯作者：
Taniguchi, Koki
中科院分区：
文献类型：
--
作者：
Ishikawa, Kumiko;Sasaki, Jun;Taniguchi, Koki
与人类急性胃肠炎相关的爱知病毒（AiV）是小核糖核酸病毒科科（Picornaviridae）的科布病毒属（Kobuvirus）的成员。小核糖核酸病毒基因组复制发生在包括病毒非结构蛋白、宿主蛋白和病毒RNA的复制复合物中。在脊髓灰质炎病毒中，所有非结构蛋白都存在于复制复合物中，这表明病毒非结构蛋白能够相互作用。在这项研究中，我们研究了AIV的非结构蛋白之间的相互作用，使用哺乳动物双杂交系统。结果表明，所有的测试蛋白质可以与一个以上的蛋白质相互作用。我们观察到同源二聚化的5个蛋白质，双向异源二聚化的6个蛋白质对，和单向异源二聚化的18个蛋白质对。在本研究中检测到的相互作用中，2A-2B、2A-2BC、2A-2C、2BC-3CD、2BC-3C、2C-3C、2C-3CD和3AB-3C相互作用在先前与其他小RNA病毒的双杂交研究中未观察到。在2A和3CD之间观察到最强的相互作用。AIV 2A已经被证明参与基因组复制。哺乳动物双杂交分析中2A和3CD相互作用的结构域作图显示，2A的C-末端四分之一不需要与3CD相互作用。(c)2009爱思唯尔有限公司版权所有。
Aichi virus (AiV), which is associated with acute gastroenteritis in humans, is a member of the genus Kobuvirus of the family Picornaviridae. Picornavirus genome replication occurs in replication complexes that include viral nonstructural proteins, host proteins and viral RNA. In poliovirus, all nonstructural proteins are found in the replication complexes, suggesting the ability of the viral nonstructural proteins to interact with each other. In this study, we examined the interactions between the AiV nonstructural proteins using a mammalian two-hybrid system. The results showed that all of the tested proteins could interact with more than one protein. We observed homodimerization of five proteins, bidirectional heterodimerization of six protein pairs, and unidirectional heterodimerization of eighteen protein pairs. Among the interactions detected in this study, the 2A-2B, 2A-2BC, 2A-2C, 2BC-3CD, 2BC-3C, 2C-3C, 2C-3CD and 3AB-3C interactions have not been observed in the previous two-hybrid studies with other picornaviruses. The strongest interaction was observed between 2A and 3CD. AiV 2A has already been shown to be involved in genome replication. Domain mapping of the 2A and 3CD interaction in mammalian two-hybrid analysis revealed that the C-terminal quarter of 2A is not required for the interaction with 3CD. (c) 2009 Elsevier B.V. All rights reserved.