SPONTANEOUS HISTAMINE-RELEASE AFTER EXPOSURE TO HYPEROSMOLAR SOLUTIONS
SPONTANEOUS HISTAMINE-RELEASE AFTER EXPOSURE TO HYPEROSMOLAR SOLUTIONS
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DOI：
10.1016/0091-6749(86)90114-4
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发表时间：
1986-07-01
影响因子：
14.2
通讯作者：
EGGLESTON, PA
中科院分区：
文献类型：
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作者：
BANKS, JR;KAGEYSOBOTKA, A;EGGLESTON, PA
由于某些过敏性个体的嗜碱性粒细胞在含水或含氘的缓冲液中自发释放组胺，我们研究了短暂暴露于高渗缓冲液后的自发释放。用来自71名正常志愿者和过敏志愿者的白细胞，发现在770 mosm/kg无Ca++缓冲液中悬浮15分钟可使细胞释放3%至83%的细胞组胺（平均29 ± 0.5%）。3）当细胞重悬于含Ca++的等渗缓冲液中时。与正常志愿者的细胞相比，来自有食物过敏史的个体的细胞明显更容易被激活（p < 0.005），但来自其他过敏志愿者的细胞也更容易被激活。激活是最大的770 mosm/kg，并发生在Ca++的情况下，而随后的组胺释放是部分Ca++依赖性的。激活可以观察到早在30秒，并在15分钟时达到最大值;组胺释放从激活的细胞几乎一样快。我们的结论是，嗜碱性粒细胞从某些过敏性个人表现出不寻常的“可释放性”，这种反应渗透激活可能发挥作用的反应。
Since basophils from certain allergic individuals release histamine spontaneously in aqueous or deuteriumoxide-containing buffers, we examined spontaneous release after a brief exposure to hyperosmolar buffer. With leukocytes from 71 normal and allegic volunteers, it was found that 15-minute suspension in 770 mosm/kg Ca++-free buffers caused the cells to release 3% to 83% of cellular histamine (mean 29 .+-. 3 ) when the cells were resuspended isosmolar buffers containing Ca++. The cells from individuals, with a history of food allergy were significantly more easily activated when the cells were compared to cells of normal volunteers (p < 0.005), but cells from other allergic volunteers were more readily activated as well. Activation was maximal at 770 mosm/kg and occurred in the absence of Ca++, whereas subsequent histamine release was partially Ca++ dependent. Activation could be observed as early as 30 seconds and was maximal at 15 minutes; histamine release from activated cells was almost as rapid. We conclude that the basophils from certain allergic individuals demonstrate unusual "releasability" and that this responsiveness to osmotic activation could play a role in reactions.