Characterization of oligosaccharides from the chondroitin sulfates -: 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides
Characterization of oligosaccharides from the chondroitin sulfates -: 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides
复制标题
DOI：
10.1046/j.1432-1327.2001.01948.x
复制
发表时间：
2001-03-01
期刊：
影响因子：
--
通讯作者：
Weeks, SD
中科院分区：
文献类型：
--
作者：
Huckerby, TN;Lauder, RM;Weeks, SD
使用酶硫酸软骨素ABC内切酶和软骨素ACII裂解酶将硫酸软骨素片段化;在还原成相应的2-脱氧-2-N-乙酰氨基-D-半乳糖醇（GalNAc-醇）形式后分离二糖和四糖片段。它们具有以下结构：Delta UA（beta1-3）GalNAc4S-ol、Delta UA（beta1-3）GalNAc6S-ol、Delta UA2S（beta1-3）GalNAc6S-ol、Delta UA（beta1-3）GalNAc4S（beta1-4）L-IdoA（α 1 -3）GalNAc4S-ol，Delta UA（β 1 -3）GalNAc4S（β 1 -4）GlcA（β 1 -3）GalNAc4S-ol，Delta UA（β 1 -3）GalNAc6S（β 1 -4）GlcA（β 1 -3）GalNAc4S-ol，Δ UA（β 1 -3）GalNAc6S（β 1 -4）GlcA（β 1 -3）GalNAc6S-ol、Δ UA2S（β 1 -3）GalNAc6S（β 1 -4）GlcA（β 1 -3）GalNAc4S-ol和Δ UA2S（β 1 -3）GalNAc6S（β 1 -4）GlcA（β 1 -3）GalNAc6S-ol，其中Δ UA表示4，5-不饱和己糖醛酸（4-脱氧-α-L-苏型-己-4-烯吡喃糖醛酸）和6S/4S/2S表示C6/C4/C2位点处的O-酯硫酸酯基团。完整的H-1-NMR和C-13-NMR数据来自这些物种，这可能有助于减轻一些显着的困难，导致信号的复杂性，目前阻碍了硫酸软骨素和硫酸皮肤素聚合物内的主要和次要结构成分的表征和分配。
Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-D-galactitol (GalNAc-ol) form. These have the structures: Delta UA(beta1-3)GalNAc4S-ol, Delta UA(beta1-3)GalNAc6S-ol, Delta UA2S(beta1-3)GalNAc6S-ol, Delta UA(beta1-3)GalNAc4S(beta1-4)L-IdoA(alpha1-3)GalNAc4S-ol, Delta UA(beta1-3)GalNAc4S(beta1-4)GlcA(beta1-3)GalNAc4S-ol, Delta UA(beta1-3)GalNAc6S(beta1-4)GlcA(beta1-3)GalNAc4S-ol, Delta UA(beta1-3)GalNAc6S(beta1-4)GlcA(beta1-3)GalNAc6S-ol, Delta UA2S(beta1-3)GalNAc6S(beta1-4)GlcA(beta1-3)GalNAc4S-ol and Delta UA2S(beta1-3)GalNAc6S(beta1-4)GlcA(beta1-3)GalNAc6S-ol, where Delta UA represents a 4,5-unsaturated hexuronic acid (4-deoxy-alpha -L-threo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete H-1-NMR and C-13-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.