Extensive amino acid polymorphism at the pgm locus is consistent with adaptive protein evolution in Drosophila melanogaster.
Extensive amino acid polymorphism at the pgm locus is consistent with adaptive protein evolution in Drosophila melanogaster.
复制标题
pgm 位点的广泛氨基酸多态性与果蝇的适应性蛋白质进化一致。
DOI：
10.1093/genetics/156.4.1737
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Eanes,WF
中科院分区：
文献类型：
--
作者：
Verrelli,BC;Eanes,WF
PGM在导致糖原代谢的分支点糖酵解途径中起核心作用，在许多物种的同工酶研究中具有高度多态性。我们已经在果蝇和果蝇d中鉴定了pgm基因的核苷酸多样性。同时研究蛋白质多态性在这个与其他几种酶共享的关键代谢分支点上的作用。AlthoughD。melanogasterandD。拟象具有共同的同工酶迁移等位基因，我们发现这些同工酶是在核苷酸水平上许多不同氨基酸变化的结果。此外，物种内的特定同工酶类包含几个氨基酸变化，这可能解释了PGM同工酶等位基因没有纬度曲线，PGM同工酶与世界蛋白（3L）Pinversion缺乏关联，以及在功能研究中未能检测到PGM同工酶之间的差异。我们发现了大量的氨基酸多态性。与完全没有固定替代物的d。simulans。在pgmlocus的2354 bp上也存在强烈的连锁不平衡，这可能是由一种特定的氨基酸单倍型解释的，这种单倍型频率很高，但包含了过多的单态多态性。与g6pd一样，pgma是一种分支点酶，具有适应性蛋白进化。
PGM plays a central role in the glycolytic pathway at the branch point leading to glycogen metabolism and is highly polymorphic in allozyme studies of many species. We have characterized the nucleotide diversity across thePgmgene inDrosophila melanogasterandD. simulansto investigate the role that protein polymorphism plays at this crucial metabolic branch point shared with several other enzymes. AlthoughD. melanogasterandD. simulansshare common allozyme mobility alleles, we find these allozymes are the result of many different amino acid changes at the nucleotide level. In addition, specific allozyme classes within species contain several amino acid changes, which may explain the absence of latitudinal clines for PGM allozyme alleles, the lack of association of PGM allozymes with the cosmopolitanIn(3L)Pinversion, and the failure to detect differences between PGM allozymes in functional studies. We find a significant excess of amino acid polymorphisms withinD. melanogasterwhen compared to the complete absence of fixed replacements withD. simulans.There is also strong linkage disequilibrium across the 2354 bp of thePgmlocus, which may be explained by a specific amino acid haplotype that is high in frequency yet contains an excess of singleton polymorphisms. LikeG6pd, Pgmshows strong evidence for a branch point enzyme that exhibits adaptive protein evolution.