Control of membrane biofouling by silver nanoparticles using Pseudomonas aeruginosa as a model bacterium
Control of membrane biofouling by silver nanoparticles using Pseudomonas aeruginosa as a model bacterium
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DOI：
10.1080/19443994.2012.698804
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发表时间：
2012-10-01
影响因子：
1.1
通讯作者：
Mamane, Hadas
中科院分区：
文献类型：
--
作者：
Dror-Ehre, Avital;Adin, Avner;Mamane, Hadas
水系统中的生物污垢导致能量损失和潜在污染。特别是在膜处理中，它通常会导致通量下降并增加对清洁程序的耐受性。研究了将细菌细胞暴露于银纳米颗粒对其在生物膜形成的初始阶段的附着以及对超滤膜装置中的生物污垢积累的缓和的影响。使用高通量筛选方法评估暴露于39 μ g/mL Ag-NP的铜绿假单胞菌细胞的初始细菌附着和生物膜形成，并与未暴露的细胞进行比较，孵育2-48小时。结果显示，与对照细胞相比，Ag-NPs稳定地延迟生物膜形成，其中附着的生物量随着孵育时间增加。为了阐明Ag-NP对UF装置中生物污垢累积的影响，检查了两个程序：（a）在生长培养基的顺序过滤之前，将预先暴露于Ag-NP的细胞通过膜过滤，和（B）在存在或不存在Ag-NP的情况下，将细胞和生长培养基的悬浮液通过膜过滤。与相应对照相比，两种程序中暴露的样品导致较低的通量下降。过滤后膜的扫描电子显微照片显示对照膜上的生物膜层和Ag-NP暴露膜上分散的单个细胞。
Biofouling in water systems results in energy loss and potential contamination. In membrane treatments in particular, it often causes flux decline and increases tolerance to cleaning procedures. The effects of exposing bacterial cells to silver nanoparticles on their attachment in the initial stage of biofilm formation and on moderating biofouling buildup in an ultrafiltration membrane apparatus were studied. A high throughput screening method was used for assessing initial bacterial attachment and biofilm formation for P. aeruginosa cells exposed to 39 mu g/mL of Ag-NPs and compared to non-exposed cells, incubated for 2-48 h. Results showed that Ag-NPs steadily retarded biofilm formation, compared to control cells, where the attached biomass increased over incubation time. To elucidate the influence of Ag-NPs on biofouling buildup in an UF apparatus, two procedures were examined: (a) cells, pre-exposed to Ag-NPs were filtered through a membrane prior to sequential filtration of growth medium, and (b) suspension of cells and growth medium were filtered through a membrane in the presence or absence of Ag-NPs. Exposed samples in both procedures resulted in lower flux decline compared to respective controls. Scanning electron micrographs of post-filtered membranes showed a biofilm layer on the control membrane and scattered individual cells on Ag-NP-exposed membrane.