Pigment composition of the photosynthetic membrane and reaction center of the green bacterium Prosthecochloris aestuarii
Pigment composition of the photosynthetic membrane and reaction center of the green bacterium Prosthecochloris aestuarii
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绿色细菌河口原绿藻光合膜和反应中心的色素组成
DOI：
10.1016/0005-2728(86)90163-5
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Amesz
中科院分区：
文献类型：
--
作者：
T. Braumann;H. Vasmel;L. Grimme;J. Amesz
我们使用反相高效液相色谱的分辨率对绿硫细菌假绿chloris aestuarii膜中存在的不同色素蛋白复合物的色素组成进行了定量分析。最纯净的光化学活性复合物仅含有类胡萝卜素（oh -氯酸钠和红紫红质）、细菌叶绿素和一种叶绿素色素，在663和433 nm处吸收最大，如细菌叶绿素。然而，这种标记为BChl 663的色素的亲脂性相当高，表明与最亲脂的BChl色素相比，它含有5-6个额外的亚甲基。比较各种色素蛋白复合物中BChl 663的含量表明，BChl 663在每个反应中心的含量为10-15分子。BChl 663的体内吸收波长为670 nm，比消光系数为85（±10）mM−1·cm−1。鉴于有证据表明初级电子受体p。在670 nm处最大吸收的一种色素(Nuijs， A.M, Vasmel， H., Joppe, h.l.p., Duysens， L.N.M. and Amesz， J.（1985）生物化学。Biophys。这些实验的一个直接结果是，只有BChl 663可能是初级电子受体的候选者，因为从这种绿色细菌的膜中提取的光化学活性色素-蛋白质复合体中没有其他吸收670 nm左右的色素（例如，噬菌体）。
We have performed a quantitative analysis of the pigment composition of different pigment-protein complexes present in the membrane of the green sulfur bacteriumProsthecochloris aestuarii, using the resolving power of reversed-phase high-performance liquid chromatography. The most purified photochemically active complexes contained only carotenoids (OH-chlorobactene and rhodopin), bacteriochlorophyllaand a chlorophyllous pigment with absorption maxima at 663 and 433 nm, like bacteriochlorophyllc. However, the lipophilicity of this pigment, labeled BChl 663, is quite high and indicates that it contains 5–6 additional methylene groups compared to the BChlchomologue known as most lipophilic. Comparison of the BChl 663 content of various pigment-protein complexes indicates that BChl 663 is present in an amount of 10–15 molecules per reaction center. BChl 663 absorbs at 670 nm in vivo, with a specific extinction coefficient of 85 (±10) mM−1· cm−1. In view of the evidence that the primary electron acceptor inP. aestuariiis a pigment with absorption maximum at 670 nm (Nuijs, A.M., Vasmel, H., Joppe, H.L.P., Duysens, L.N.M. and Amesz, J. (1985) Biochim. Biophys. Acta 807, 24–34) a direct consequence of these experiments is the fact that only BChl 663 can be a likely candidate for the role of primary electron acceptor as no other pigments absorbing around 670 nm (e.g., bacteriopheophytinc) are present in a photochemically active pigment-protein complex derived from the membrane of this green bacterium.