Artificial insemination using cryopreserved sperm in the silkworm, Bombyx mori.
Artificial insemination using cryopreserved sperm in the silkworm, Bombyx mori.
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使用家蚕冷冻精子进行人工授精。
DOI：
10.1016/s0022-1910(99)00135-3
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发表时间：
2000
影响因子：
2.2
通讯作者：
Y. Horie
中科院分区：
文献类型：
--
作者：
Y. Takemura;T. Kanda;Y. Horie
本文报道了用冷冻精子(−-196℃)人工授精的雌蛾，其精子保存356天后可产卵，其受精率和产卵量与正常交配的雌蛾相当。为保持精子的高受精率，冷冻精子的最佳降温速率为5~65℃/min。简单可靠的冷冻方法是先将精液放入−80°C的深度冷冻箱中，然后将其放入液氮中。当‘白色2’卵色突变品系的雌蛾用正常卵系雄蛾的冻融精子和‘白色2号’雄蛾的未冷冻精子的混合物授精时，雌蛾产下了大部分的‘白色2’卵和极少量的正常颜色的卵。结果表明，两个品系家蚕精子之间存在竞争性受精，−-196℃冷冻可显著降低精子受精率。这一结果不仅为鳞翅目昆虫受精的基础研究提供了依据，也为利用家蚕精子冷冻进行遗传资源长期保存的研究奠定了基础。
We report in this paper that female moths artificially inseminated with cryopreserved sperm (−196°C) could oviposit eggs when the sperm was preserved for 356days, and that the fertilization rate and the number of eggs laid were almost equivalent to those obtained in normally mated moths. The optimal cooling rate for sperm freezing was 5–65°C/min for maintaining a high fertility of sperm. The simple and reliable method of cryopreservation was to put the semen first in a deep freezer at −80°C and thereafter put them in liquid nitrogen. When female moths of ‘white 2’ egg-color mutant strain were inseminated with a mixture of frozen–thawed sperm from males of normal-colored egg strain and non-frozen sperm from males of the ‘white 2’, female moths deposited a majority of ‘white 2’ eggs and a very small number of eggs of normal color. The result shows that there was a competitive fertilization of sperm between the two strains of the silkworm, and that sperm fertility was reduced to a considerable extent by freezing at −196°C. These results may contribute not only to basic studies on fertilization in Lepidoptera but also to the development of long-term preservation procedure of genetic resources by using cryopreserved sperm of Bombyx mori.