Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins
Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins
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DOI：
10.1006/jmre.1999.1860
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发表时间：
1999-10-01
影响因子：
2.2
通讯作者：
Sorensen, OW
中科院分区：
文献类型：
--
作者：
Meissner, A;Sorensen, OW
提出了一种抑制N-15标记蛋白质NOESY核磁共振酰胺区对角峰的新方法。该方法对于在高磁场下的较大蛋白质特别有用，其中偶极和化学位移各向异性弛豫机制之间的干扰导致大的TROSY效应，即，H-1（N）线宽的巨大差异取决于附着的N-15核的自旋状态。在此限度内，新的TROSY NOESY方法不会影响灵敏度。使用全氘代N-15标记的蛋白质样品（来自大鼠的神经细胞粘附分子213-308（NCAM））在800 MHz的H2O中进行了证明。(C)北京：科学出版社.
A novel method for suppression of diagonal peaks in the amide region of NOESY NMR spectra of N-15-labeled proteins is presented. The method is particularly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation mechanisms results in large TROSY effects, i.e., large differences in H-1(N) Linewidths depending on the spin state of attached N-15 nuclei. In this limit the new TROSY NOESY method does not compromise sensitivity, It is demonstrated using a perdeuterated N-15-labeled protein sample, Neural Cell Adhesion Molecule 213-308 (NCAM) from rat, in H2O at 800 MHz. (C) 1999 Academic Press.