Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone
Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone
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DOI：
10.2108/zs130265
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发表时间：
2014-05
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影响因子：
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通讯作者：
Koji Yachiguchi;T. Sekiguchi;Masaki Nakano;A. Hattori;Megumi Yamamoto;K. Kitamura;M. Maeda;Y. Tabuchi;T. Kondo;H. Kamauchi;H. Nakabayashi;A. Srivastav;K. Hayakawa;T. Sakamoto;N. Suzuki
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作者：
Koji Yachiguchi;T. Sekiguchi;Masaki Nakano;A. Hattori;Megumi Yamamoto;K. Kitamura;M. Maeda;Y. Tabuchi;T. Kondo;H. Kamauchi;H. Nakabayashi;A. Srivastav;K. Hayakawa;T. Sakamoto;N. Suzuki
为评价无机汞(InHg)和甲基汞(MeHg)对海水硬骨鱼骨代谢的影响，分别检测了破骨细胞和成骨细胞中抗酒石酸酸性磷酸酶(TRAP)和碱性磷酸酶(ALP)的活性。我们发现了几条鳞片，它们的TRAP和ALP活性水平几乎相同。使用这些量表，我们评估了汞和甲基汞的影响。汞(10-5和10-4M)和甲基汞(10-6到10-4M)处理的鳞片在孵育6h内TRAP活性显著降低。与之相反，碱性磷酸酶活力在接触汞(10-5和10-4M)和甲基汞(10-6到10-4M)18和36小时后下降，但在孵育6小时后其活性没有变化。在酶活性方面，InHg和MeHg作用6h后，破骨细胞标志物TRAP的mRNA表达显著降低，而成骨细胞标志物胶原的mRNA表达无明显变化。在孵育6h后，成骨细胞中金属结合蛋白金属硫蛋白的mRNA表达明显增加，提示其可能参与了成骨细胞对汞暴露的保护作用。据我们所知，这是首次报道汞对破骨细胞和成骨细胞的影响，使用海洋硬骨鱼作为骨的模型系统。
To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10-5 and 10-4 M) and MeHg (10-6 to 10-4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10-5 and 10-4 M) and MeHg (10-6 to 10-4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.