INDUCED MESSENGER-RNA STABILITY IN BACILLUS-SUBTILIS
INDUCED MESSENGER-RNA STABILITY IN BACILLUS-SUBTILIS
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DOI：
10.1073/pnas.84.2.498
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发表时间：
1987-01-01
影响因子：
11.1
通讯作者：
DUBNAU, D
中科院分区：
文献类型：
--
作者：
BECHHOFER, DH;DUBNAU, D
我们研究了枯草芽孢杆菌ermC基因编码的mRNA的诱导稳定性。已知红霉素诱导ermC基因表达发生在翻译水平。我们发现，这种诱导是伴随着ermC mRNA的半衰期从约2分钟增加到约40分钟。诱导的ermC mRNA的稳定化发生独立的诱导翻译。稳定性所需的调控序列是启动子近端的，并且可以赋予具有不同3“末端的大mRNA诱导的稳定性。ermC前导肽的翻译和前导肽序列中的核糖体停滞是诱导稳定所必需的。
We have investigated the induced stability of mRNA encoded by the ermC gene in Bacillus subtilis. Induction of ermC gene expression by erythromycin is known to occur at the translational level. We show that this induction is accompanied by an increase in ermC mRNA half-life from about 2 min to about 40 min. Induced stabilization of ermc mRNA occurs independently of induced translation. The regulatory sequences required for stability are promoter-proximal and can confer induced stability on large mRNAs having diverse 3'' ends. Translation of the ermC leader peptide and ribosome-stalling in the leader peptide sequence are necessary for induced stabilization.