Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing
Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing
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DOI：
10.1117/1.jbo.19.10.101506
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发表时间：
2014-10-01
影响因子：
3.5
通讯作者：
Loehmannsroeben, Hans-Gerd
中科院分区：
文献类型：
--
作者：
Cywinski, Piotr J.;Hammann, Tommy;Loehmannsroeben, Hans-Gerd
已经使用硒化镉量子点（QD）、铕络合物（EuCs）和生物素合成了纳米生物缀合物。在这些缀合物中，长寿命的光致发光（PL）由铕络合物提供，其通过福斯特共振能量转移（FRET）有效地将能量转移到空间接近的QD。结果，缀合物具有QD的PL发射光谱特性和EuCs的长PL衰减时间特性。描述了纳米生物缀合物的合成策略和光物理性质以及它们在时间分辨的链霉亲和素-生物素PL测定中的性能。为了制备QD-EuC-生物素缀合物，首先用EuC和生物素官能化两亲聚合物。然后，将聚合物带到QD（QD 655或QD 705）的表面上以提供功能性并使QD可水分散。由于EuC和QD之间的短距离，可以观察到有效的FRET。此外，QD-EuC-生物素缀合物的功能性已经在PL测定中得到证实，产生良好的信号区分，来自自发荧光和直接激发的QD。这些新设计的QD-EuC-生物素缀合物扩展了用于诊断和成像应用的生物分析光学检测方法的高灵敏度工具的类别。(C)2014年，美国光电仪器工程师学会（SPIE）
Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Forster resonance energy transfer (FRET) to the QDs in close spatial proximity. As a result, the conjugates have a PL emission spectrum characteristic for QDs combined with the long PL decay time characteristic for EuCs. The nanobioconjugates synthesis strategy and photo-physical properties are described as well as their performance in a time-resolved streptavidin-biotin PL assay. In order to prepare the QD-EuC-biotin conjugates, first an amphiphilic polymer has been functionalized with the EuC and biotin. Then, the polymer has been brought onto the surface of the QDs (either QD655 or QD705) to provide functionality and to make the QDs water dispersible. Due to a short distance between EuC and QD, an efficient FRET can be observed. Additionally, the QD-EuC-biotin conjugates' functionality has been demonstrated in a PL assay yielding good signal discrimination, both from autofluorescence and directly excited QDs. These newly designed QD-EuC-biotin conjugates expand the class of highly sensitive tools for bioanalytical optical detection methods for diagnostic and imaging applications. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)