A vector for expressing foreign genes in the brains and hearts of transgenic mice
A vector for expressing foreign genes in the brains and hearts of transgenic mice
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DOI：
10.1016/s1050-3862(96)00167-2
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发表时间：
1996-12-01
期刊：
影响因子：
--
通讯作者：
Price, DL
中科院分区：
文献类型：
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作者：
Borchelt, DR;Davis, J;Price, DL
从启动子，5'内含子和3'非翻译序列中开发出一种用于转基因小鼠的表达质粒（Moprp.xho）。用编码编码淀粉样蛋白前体蛋白（APP）和人类presenilin 1的cDNA基因的小鼠的分析表明，该载体在转基因小鼠的大脑和心脏中提供了相对较高的转基因编码多肽。 Moprp.xho载体在旨在过表达各种野生型和疾病与心脏和大脑中的野生型和疾病相关的突变基因的策略中应该非常有用。 （c）1996 Elsevier Science B.V.
An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5' intronic, and 3' untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin 1 demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain. (C) 1996 Elsevier Science B.V.