Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs
Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs
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DOI：
10.4172/2157-2518.1000230
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
H. Ichihara;Y. Komizu;R. Ueoka;Y. Matsumoto
中科院分区：
文献类型：
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作者：
H. Ichihara;Y. Komizu;R. Ueoka;Y. Matsumoto
本文研究了由90 mol% L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）和10 mol%聚氧乙烯（23）十二烷基醚（C12（EO）23）组成的混合脂质体（HL）对人肺癌细胞（A549）神经酰胺生成和侵袭抑制作用。通过激活中性鞘磷脂酶，观察HL处理的A549细胞的神经酰胺生成。这是HL处理后A549细胞中鞘磷脂的减少。HL对A549细胞的抗侵袭作用是通过抑制MT 1 MMP/MMP 14而实现的。采用划痕法观察HL对A549细胞迁移的抑制作用。
Ceramide generation and invasive inhibition by hybrid liposomes (HL) composed of 90 mol% L-α-dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (23) dodecyl ether (C12(EO)23) were examined for human lung cancer (A549) cells in vitro. Ceramide generation for A549 cells treated with HL was observed through the activation of neutral sphingomyelinase. These was a reduction in sphingomyelin in A549 cells after treatment with HL. The anti-invasive effects of HL for A549 cells were obtained through the inhibition of MT1MMP/MMP14. Inhibitory effects of HL on the migration of A549 cells was obtained using scratch assay.