Role of Adult Tissue-Derived Pluripotent Stem Cells in Bone Regeneration
Role of Adult Tissue-Derived Pluripotent Stem Cells in Bone Regeneration
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DOI：
10.1007/s12015-019-09943-x
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发表时间：
2020-02-01
影响因子：
4.8
通讯作者：
Barker, J. H.
中科院分区：
文献类型：
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作者：
Leppik, Liudmila;Sielatycka, K.;Barker, J. H.
骨髓来源的单个核细胞（BM-MNC）由间充质干细胞（MSC）、造血祖细胞（HPC）、内皮祖细胞（EPC）、单核细胞、淋巴细胞和多能干细胞组成。尽管MSC和EPC在骨愈合和再生研究中的重要性已经得到了充分的证明，但多能干细胞的作用仍然知之甚少。在本研究中，我们评估了从大鼠BM-MNC中分离的非常小的胚胎样细胞（VSEL）是否以及如何促进骨愈合。方法38只Sprague Dawley雌性大鼠股骨造大骨缺损，用β - tcp支架颗粒植入雄性VSEL；术后8周评估BM-MNC、vsel缺失BM-MNC或单独支架的骨愈合情况。结果与VSEL缺失的BM-MNC相比，VSEL和BM-MNC治疗的缺损骨愈合明显增加。供体细胞存在于新生骨组织中，存在于所有用细胞处理过的缺损中，仅存在于用vsel缺失的BM-MNC处理过的缺损的纤维组织中。CD68+细胞数量以vsel缺失组最多，TRAP阳性细胞数量以vsel缺失组最少。结论VSEL在bmm - mnc诱导的骨形成中起一定作用。在我们的大鼠股骨缺损模型中，用vsel缺失的BM-MNC处理缺损后，破骨细胞发生和骨形成减少，异物反应增加。
BackgroundBone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone healing and regeneration studies, the role of pluripotent stem cells is still poorly understood. In the present study we evaluated if and how Very Small Embryonic Like cells (VSEL), isolated from rat BM-MNC, contribute to bone healing.MethodsLarge bone defects were made in the femurs of 38 Sprague Dawley female rats and treated with beta-TCP scaffold granules seeded with male VSEL; BM-MNC, VSEL-depleted BM-MNC or scaffold alone, and bone healing was evaluated at 8 weeks post-surgery.ResultsBone healing was significantly increased in defects treated with VSEL and BM-MNC, compared to defects treated with VSEL-depleted BM-MNC. Donor cells were detected in new bone tissue, in all the defects treated with cells, and in fibrous tissue only in defects treated with VSEL-depleted BM-MNC. The number of CD68+ cells was the highest in the VSEL-depleted group, whereas the number of TRAP positive cells was the lowest in this group.ConclusionsBased on the results, we can conclude that VSEL play a role in BM-MNC induced bone formation. In our rat femur defect model, in defects treated with VSEL-depleted BM-MNC, osteoclastogenesis and bone formation were decreased, and foreign body reaction was increased.