Are there predominant strains and toxins of Staphylococcus aureus in atopic dermatitis patients? Genotypic characterization and toxin determination of S-aureus isolated in adolescent and adult patients with atopic dermatitis
Are there predominant strains and toxins of Staphylococcus aureus in atopic dermatitis patients? Genotypic characterization and toxin determination of S-aureus isolated in adolescent and adult patients with atopic dermatitis
复制标题
DOI：
10.1111/j.1346-8138.2009.00592.x
复制
发表时间：
2009-02-01
影响因子：
3.1
通讯作者：
Kim, Jungmin
中科院分区：
文献类型：
--
作者：
Kim, Do-Won;Park, Jee-Young;Kim, Jungmin
金黄色葡萄球菌的定植是特应性皮炎（AD）最重要的加重因素之一。到目前为止，金黄色葡萄球菌在AD中的重要性以及金黄色葡萄球菌的定植与临床严重程度/皮肤屏障功能之间的正相关已经得到证实。本研究的目的是确定是否有特定的金黄色葡萄球菌克隆定植在AD患者的皮肤上。为此，采用新开发的分型方法对从AD患者分离的金黄色葡萄球菌进行基因型检测。对35例AD患者分离的36株金黄色葡萄球菌进行spa分型、多位点序列分型（MLST）和多重聚合酶链反应（pcr）法进行葡萄球菌毒素基因检测。用湿疹面积及严重程度指数（EASI）和经皮失水（TEWL）评价临床严重程度和皮肤屏障功能。在36株金黄色葡萄球菌中，鉴定出14种序列型（ST）和20种spa型，表明金黄色葡萄球菌的遗传组成非常异质性，并且在AD患者定殖的金黄色葡萄球菌中缺乏流行的基因型。此外，金黄色葡萄球菌没有特定的基因型与AD的临床严重程度或皮肤屏障功能障碍相关。然而，毒素基因测定显示，金黄色葡萄球菌菌株携带sea和/或tst -1的优势。据我们所知，这是第一个用基于序列的分型方法确定从AD患者分离的金黄色葡萄球菌菌株的遗传组成的报告。
The colonization of Staphylococcus aureus is one of the most important aggravating factors of atopic dermatitis (AD). Until now, the importance of S. aureus in AD and a positive correlation between colonization with S. aureus and clinical severity/skin barrier function has been demonstrated. The aim of this study was to determine whether there are certain clones of S. aureus which colonize the skin of AD patients. For this purpose, the genotype of S. aureus isolated from AD patients was examined by newly-developed typing methods. With 36 strains of S. aureus isolated from 35 patients with AD, spa typing, multi-locus sequence typing (MLST), and staphylococcal toxin gene assay by multiplex polymerase chain reaction, were performed. Clinical severity and skin barrier function were evaluated with eczema area and severity index (EASI) and with transepidermal water loss (TEWL). Among 36 strains of S. aureus, 14 sequence types (ST) and 20 spa types were identified, suggesting a very heterogeneous genetic composition of S. aureus and the absence of a prevailing genotype in S. aureus colonized with AD patients. Furthermore, there was no specific genotype of S. aureus which was associated with the clinical severity of AD or skin barrier dysfunction. A toxin gene assay, however, showed the predominance of S. aureus strains carrying sea and/or tsst-1. To the best of our knowledge, this is the first report to show the genetic composition of S. aureus strains isolated from AD patients determined by sequence-based typing methods.