Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.
Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.
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DOI：
10.1038/srep44522
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发表时间：
2017-03-16
影响因子：
4.6
通讯作者：
Kuboki T
中科院分区：
文献类型：
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作者：
Ono M;Oshima M;Ogawa M;Sonoyama W;Hara ES;Oida Y;Shinkawa S;Nakajima R;Mine A;Hayano S;Fukumoto S;Kasugai S;Yamaguchi A;Tsuji T;Kuboki T
全器官再生具有巨大的潜力，可以通过体外三维细胞操作重建功能齐全的生物工程器官来替代功能失调的器官。近年来，在小鼠模型上进行了许多三维细胞操作全牙替代的基础研究。需要进一步的证据来证明在人类医学上的实际应用，通过在出生后的大型动物模型上重建生物工程牙胚来证明牙齿修复。在此，我们通过在出生后犬模型中自体移植生物工程牙胚来展示牙齿功能修复。利用恒牙生殖细胞重建的生物工程牙，在自体移植后突入颌骨，具有与天然牙相当的生理功能。该研究代表了通过生物工程器官胚移植作为未来临床再生医学实用模型的全器官替代治疗的实质性进展。
Whole-organ regeneration has great potential for the replacement of dysfunctional organs through the reconstruction of a fully functional bioengineered organ using three-dimensional cell manipulation in vitro. Recently, many basic studies of whole-tooth replacement using three-dimensional cell manipulation have been conducted in a mouse model. Further evidence of the practical application to human medicine is required to demonstrate tooth restoration by reconstructing bioengineered tooth germ using a postnatal large-animal model. Herein, we demonstrate functional tooth restoration through the autologous transplantation of bioengineered tooth germ in a postnatal canine model. The bioengineered tooth, which was reconstructed using permanent tooth germ cells, erupted into the jawbone after autologous transplantation and achieved physiological function equivalent to that of a natural tooth. This study represents a substantial advancement in whole-organ replacement therapy through the transplantation of bioengineered organ germ as a practical model for future clinical regenerative medicine.