Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol
Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol
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DOI：
10.1128/aem.00468-08
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发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Liao, James C.
中科院分区：
文献类型：
--
作者：
Connor, Michael R.;Liao, James C.
3-甲基-1-丁醇是一种潜在的燃料添加剂或替代品。以前，这种化合物在酵母发酵中少量被鉴定为杂醇醇之一。在这项工作中，我们设计了一个大肠杆菌菌株，通过宿主的氨基酸生物合成途径从葡萄糖生产3-甲基-1-丁醇。通过消除反馈抑制和竞争途径的菌株改进提高了3-甲基-1-丁醇的选择性和生产率。这项工作证明了生产3-甲基-1-丁醇作为生物燃料的可行性，并显示出利用E。coli作为生产宿主。
3-Methyl-1-butanol is a potential fuel additive or substitute. Previously this compound was identified in small quantities in yeast fermentation as one of the fusel alcohols. In this work, we engineered an Escherichia coli strain to produce 3-methyl-1-butanol from glucose via the host's amino acid biosynthetic pathways. Strain improvement with the removal of feedback inhibition and competing pathways increased the selectivity and productivity of 3-methyl-1-butanol. This work demonstrates the feasibility of production of 3-methyl-1-butanol as a biofuel and shows promise in using E. coli as a host for production.