Antibiotic resistance levels in soils from urban and rural land uses in Great Britain.
Antibiotic resistance levels in soils from urban and rural land uses in Great Britain.
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DOI：
10.1099/acmi.0.000181
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发表时间：
2021
影响因子：
--
通讯作者：
Fernández-Martínez LT
中科院分区：
文献类型：
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作者：
Osbiston K;Oxbrough A;Fernández-Martínez LT
虽然土壤是最大的微生物多样性库之一，但定义其微生物群落动态的过程尚未完全了解。提高我们对英国不同土地用途土壤中抗生素耐药性水平的理解不仅对保护动物健康（包括人类）很重要，而且对深入了解微生物群落中的基因转移水平也很重要。本研究观察了能够在氯霉素、红霉素和万古霉素的抑制浓度以及亚抑制（10 µg ml−1）红霉素浓度下存活的耐药细菌（ARB）水平。从九个不同的网站在英国与三个不同的土地用途（农业，城市和半自然）的土壤进行了采样，并计算每个网站的ARB的百分比。统计分析证实，与城市或半自然地点相比，农业用地中的ARB水平存在显着差异。结果还表明，万古霉素和氯霉素的耐药性水平在农业和城市采样点显着高于红霉素，而在半自然的网站，所有三种抗生素显示出类似的耐药性水平。最后，尽管与对抑制浓度（20 µ g ml − 1）的耐药性水平相比，对亚抑制浓度（10 µg ml − 1）红霉素的耐药性水平在土地利用类型中显著更高，但与城市或半自然土地利用土壤相比，农业用地土壤中的耐药性水平要低得多。
Although soil is one of the largest microbial diversity reservoirs, the processes that define its microbial community dynamics are not fully understood. Improving our understanding of the levels of antibiotic resistance in soils with different land uses in Great Britain is not only important for the protection of animal health (including humans), but also for gaining an insight into gene transfer levels in microbial communities. This study looked at the levels of antibiotic-resistant bacteria (ARB) able to survive inhibitory concentrations of chloramphenicol, erythromycin and vancomycin, as well as subinhibitory (10 µg ml−1) erythromycin concentrations. Soils from nine different sites across Great Britain with three distinct land uses (agricultural, urban and semi-natural) were sampled and the percentage of ARB was calculated for each site. Statistical analyses confirmed a significant difference in the level of ARB found in agricultural land compared to urban or semi-natural sites. The results also showed that resistance levels to vancomycin and chloramphenicol in the agricultural and urban sites sampled were significantly higher than those for erythromycin, whilst in semi-natural sites all three antibiotics show similar resistance levels. Finally, although the levels of resistance to a subinhibitory (10 µg ml−1) erythromycin concentration were significantly higher across land use types when compared to the levels of resistance to an inhibitory (20 µg ml−1) concentration, these were much less marked in soil from agricultural land compared to that from urban or semi-natural land use soil.