CEREBRAL METABOLIC STUDIES INVIVO BY P-31 NMR
CEREBRAL METABOLIC STUDIES INVIVO BY P-31 NMR
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DOI：
10.1073/pnas.80.9.2748
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
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作者：
PRICHARD, JW;ALGER, JR;SHULMAN, RG
在具有200-mm净孔的光谱仪中，在32 MHz下对活兔的脑进行31 P NMR研究。将N2 O镇痛下的麻痹泵通气动物插入1.89-T场，并通过使用4-cm表面线圈将信号聚焦在大脑中。几个传统的生理变量与31 P光谱一起监测诱导和逆转胰岛素休克和缺氧缺氧足以废除EEG和癫痫持续状态期间。在低血糖和缺氧期间，检测到磷酸肌酸储存可逆性减少，伴随着Pi的增加。在长期癫痫持续状态中观察到类似的变化，但没有逆转。三磷酸腺苷水平下降. apprx。在低血糖中为50%，但在其他2种代谢应激中仅为轻微。低血糖时细胞内pH值升高;癫痫持续状态和缺氧时pH值下降，但仅在心血管功能严重受损时才出现。从测量的NMR参数和假设，肌酸激酶处于平衡状态，肌酸/磷酸肌酸池是恒定的，这是可能的，以计算与这些代谢紊乱相关的细胞质ADP水平的相对变化。
31P NMR studies on the brains of living rabbits were carried out at 32 MHz in a spectrometer having a 200-mm clear bore. Paralyzed pump-ventilated animals under N2O analgesia were inserted into the 1.89-T field and signals were focused in the brain by using a 4-cm surface coil. Several conventional physiological variables were monitored together with 31P spectra during induction and reversal of insulin shock and hypoxic hypoxia sufficient to abolish the EEG and during status epilepticus. A reversible decrease in phosphocreatine stores accompanied by an increase in Pi was detected during hypoglycemia and hypoxia. Similar changes were observed in prolonged status epilepticus but were not reversed. ATP levels fell .apprx. 50% in hypoglycemia but only slightly in the other 2 metabolic stresses. Intracellular pH rose in hypoglycemia; in status epilepticus and hypoxia it fell, but only when cardiovascular function was severely impaired. From the measured NMR parameters and the assumptions that creatine kinase was at equilibrium and that the creatine/phosphocreatine pool was constant, it was possible to calculate the relative changes in cytoplasmic ADP levels associated with these metabolic disturbances.