Salivary levels of alpha(2)-macroglobulin, alpha(1)-antitrypsin, C-reactive protein, cathepsin G and elastase in humans with or without destructive periodontal disease
Salivary levels of alpha(2)-macroglobulin, alpha(1)-antitrypsin, C-reactive protein, cathepsin G and elastase in humans with or without destructive periodontal disease
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DOI：
10.1016/0003-9969(95)00089-5
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发表时间：
1995-12-01
影响因子：
3
通讯作者：
Turner, DW
中科院分区：
文献类型：
--
作者：
Pederson, ED;Stanke, SR;Turner, DW
通过酶联免疫吸附试验对45名成年人（19名男性，26名女性）的未刺激全唾液样本进行了5项宿主反应指标的测定。参与者分布在四个牙齿组中，分别代表口腔健康（I）、牙龈炎（II）、中度牙周炎（III）和重度牙周炎（IV），以及一组无牙志愿者（V）。宿主反应指标的水平差别很大，从零（主要是第一和第五组）到相对较高的第二、第三和第四组。级别范围如下：α（2）-巨球蛋白，0-4941 ng/ml; α（1）-抗胰蛋白酶，2-2271 ng/ml; C-反应蛋白，0-472 pg/ml;组织蛋白酶G，0-6035 ng/ml;弹性蛋白酶，0-164 ng/ml（游离），0-732 ng/ml（与α（1）-抗胰蛋白酶结合）和0-318 ng/ml（与α（2）-巨球蛋白结合）。通过计划对比进行的统计学评价显示，组I的宿主应答指标水平（α（1）-抗胰蛋白酶除外）显著低于组II、III和IV。组I-IV的趋势分析显示，平均评分（同样，α（1）-抗胰蛋白酶除外）以正单调方式显著增加。组V显示弹性蛋白酶的值显著低于其他组。研究结果表明，这些因素可以在整个唾液中检测到，并表明，除了α（1）-抗胰蛋白酶，它们的水平与个人的牙周状况直接相关。
Five host-response indicators were measured by enzyme-linked immunosorbent assays on unstimulated whole saliva samples from 45 adults (19 male, 26 female). The participants were distributed among four dentate groups representing oral health (I), gingivitis (II), moderate periodontitis (III), and severe periodontitis (IV), and one group of edentulous volunteers (V). Levels of the host-response indicators varied widely, from zero, primarily with groups I and V, to relatively high values with groups II, III and IV. The levels ranged as follows: alpha(2)-macroglobulin, 0-4941 ng/ml; alpha(1)-antitrypsin, 2-2271 ng/ml; C-reactive protein, 0-472 pg/ml; cathepsin G, 0-6035 ng/ml; elastase, 0-164 ng/ml (free), 0-732 ng/ml (bound to alpha(1)-antitrypsin), and 0-318 ng/ml (bound to alpha(2)-macroglobulin). Statistical evaluation by planned contrasts showed that levels of host-response indicators for group I were significantly lower (except for alpha(1)-antitrypsin) than for groups II, III, and IV. A trend analysis of groups I-IV showed that mean scores (again, except for alpha(1)-antitrypsin) increased significantly in a positive, monotonic manner. Group V showed significantly lower values for elastase than in the other groups. The findings demonstrate that these factors can be detected in whole saliva and suggest that, except for alpha(1)-antitrypsin, their levels are directly related to an individual's periodontal status.