Population genetic structure and classification of cultivated and wild pea (Pisum sp.) based on morphological traits and SSR markers
Population genetic structure and classification of cultivated and wild pea (Pisum sp.) based on morphological traits and SSR markers
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基于形态性状和SSR标记的栽培和野生豌豆（Pisum sp.）的群体遗传结构和分类
DOI：
10.1111/jse.12710
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发表时间：
2021-04-06
影响因子：
3.7
通讯作者：
Zong, Xu-Xiao
中科院分区：
文献类型：
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作者：
Liu, Rong;Huang, Yu-Ning;Zong, Xu-Xiao
豌豆 (Pisum sativum L.) 是一种重要的豆类作物，在世界范围内广泛种植，用于人类消费和牲畜饲料。尽管进行了广泛的研究，但栽培豌豆和野生豌豆（Pisum sp.）的种群遗传结构和分类仍然存在争议。为了表征豌豆的遗传和形态变异模式并研究豌豆的分类，我们利用 34 个形态性状和 87 个多态性简单序列重复标记，对来自栽培和野生豌豆的 323 个种质（代表豌豆的 3 个物种）进行了全面的群体遗传分析。首先，我们在所有样本中确定了三个不同的遗传群体。第一组主要由豌豆、深豌豆和一些野生豌豆种质组成，而第二组和第三组由豌豆下的两个遗传组组成，代表了栽培豌豆的不同地理分布。形态变异分析揭示了这三个物种之间的显着差异。其次，在代表豌豆8个类群的豌豆种质中，黄豌豆和阿比西豌豆具有独特的遗传背景和形态特征，证实了它们的独立物种地位。除 P. sativum subsp. 的几种基因型外，本研究不支持一些作者描述的 P. sativum 种内细分。 elatius 与 P. fulvum 和 P. abyssinicum 聚集在一起。最后，我们证实中国豌豆种质在遗传上是独特的，可以分为两个遗传组，每个遗传组都包括春播和秋播生态型。这些结果为了解豌豆驯化和豌豆野生遗传资源的利用提供了坚实的基础。
Pea (Pisum sativum L.) is an important legume crop that is widely grown worldwide for human consumption and livestock feed. Despite extensive studies, the population genetic structure and classification of cultivated and wild pea (Pisum sp.) remain controversial. To characterize patterns of genetic and morphological variation and investigate the classification of Pisum, we conducted comprehensive population genetic analyses for 323 accessions from cultivated and wild pea, representing three species of Pisum and utilizing 34 morphological traits and 87 polymorphic simple sequence repeat markers. First, we identified three distinct genetic groups among all samples. Group I was primarily composed of Pisum fulvum, Pisum abyssinicum, and some wild P. sativum accessions, whereas Groups II and III consisted of the two genetic groups under P. sativum that represented different geographic distributions of cultivated pea. Analyses of morphological variation revealed significant differences among the three species. Second, among pea germplasms representing eight taxa of Pisum, P. fulvum and P. abyssinicum possessed unique genetic backgrounds and morphological characteristics, corroborating their independent species status. The intraspecific subdivisions of P. sativum described by some authors were not supported in this study, with the exception of several genotypes of P. sativum subsp. elatius that clustered with P. fulvum and P. abyssinicum. Finally, we confirmed that the Chinese pea germplasm was genetically distinct and could be divided into two genetic groups, each of which included both spring‐sowing and autumn‐sowing ecotypes. These results provide a robust foundation for understanding pea domestication and the utilization of wild genetic resources of pea.