EXPERIMENTAL CAMPYLOBACTER-JEJUNI INFECTION OF ADULT MICE
EXPERIMENTAL CAMPYLOBACTER-JEJUNI INFECTION OF ADULT MICE
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DOI：
10.1128/iai.39.2.908-916.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
WANG, WLL
中科院分区：
文献类型：
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作者：
BLASER, MJ;DUNCAN, DJ;WANG, WLL
以HA-ICR成年小鼠为研究对象，建立了空肠弯曲菌的动物模型。空肠]人类的肠炎。用选择性和非选择性方法进行的粪便和回肠培养表明，空肠弯曲菌和相关微生物不是肠道共生体。空肠弯曲菌3种不同菌株的108个集落形成单位(CFU)灌胃后，100%动物感染，感染率呈剂量依赖关系。不需要使用抗生素或阿片类药物进行预治疗来诱导感染。新鲜的菌株和在人工培养基上传递的菌株产生相似的感染率。感染的小鼠没有表现出疾病的迹象；几乎100%的小鼠在口腔感染后10分钟内观察到一过性菌血症。小肠是主要的靶器官，感染后48h可见上皮炎症。4个品系的对照小鼠血清中未检测到针对感染毒株的特异性抗体，感染小鼠在感染后1wk出现滴度高峰，并迅速下降。免疫球蛋白滴度上升幅度很小，免疫球蛋白A滴度没有上升。感染的小鼠一致成为慢性无症状排泄者，排出104-106cfu/g的粪便；少数是胆道携带者。肠道携带以胃和近端小肠最明显。由于这种实验性感染导致菌血症、一过性病变和免疫球蛋白G滴度升高，该模型可能有助于评估预防和治疗干预的效果。
HA-ICR adult mice were studied to develop an animal model for C. jejuni [C. fetus ssp. jejuni] enteritis in humans. Fecal and ileal cultures made by selective and nonselective methods showed that C. jejuni and related organisms are not bowel commensals. Intragastric feeding of 108 colony-forming units (CFU) of 3 different strains of C. jejuni produced infection in 100% of the animals; infection rates were dose dependent. Pretreatment with antibiotics or opiates was not necessary to induce infection. Fresh isolates and strains passed on artificial media yielded similar infection rates. Infected mice did not show signs of illness; transient bacteremia within 10 min of oral infection was observed in nearly 100%. The small intestine was the principal target organ, with epithelial inflammation seen 48 h after infection. Control mice of 4 strains had undetectable serum IgG antibody specific for the infecting strain; infected mice showed peak titers at 1 wk with rapid decline. IgM titers rose minimally; IgA titers did not rise. Infected mice uniformly became chronic asymptomatic excretors, shedding 104-106 CFU/g of feces; a minority were biliary carriers. Intestine carriage was most pronounced in the stomach and proximal small intestine. Because this experimental infection led to bacteremia, transient pathological changes and IgG titer rises, this model may be useful for evaluating the effects of prophylactic and therapeutic interventions.