A study of eukaryotic response mechanisms to atmospheric pressure cold plasma by using Saccharomyces cerevisiae single gene mutants
A study of eukaryotic response mechanisms to atmospheric pressure cold plasma by using Saccharomyces cerevisiae single gene mutants
复制标题
利用酿酒酵母单基因突变体研究真核生物对常压冷等离子体的响应机制
DOI：
10.1063/1.3491180
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发表时间：
2010-09-27
影响因子：
4
通讯作者：
Zhang, Jue
中科院分区：
文献类型：
--
作者：
Feng, Hongqing;Wang, Ruixue;Zhang, Jue
研究了真核细胞对冷等离子体的反应机制。用真核模式生物酿酒酵母的一系列单基因突变体与野生型比较其对血浆处理的敏感性。我们检测了氧化应激途径和细胞周期途径中的12个突变体，其中8个突变体对血浆加工敏感。分析突变基因在这两种途径中的作用，以了解血浆治疗的生物学反应机制。结果表明，这两种途径的基因都是真核细胞在复杂的血浆处理中存活所必需的。
The mechanisms of eukaryotic cell response to cold plasma are studied. A series of single gene mutants of eukaryotic model organism Saccharomyces cerevisiae are used to compare their sensitivity to plasma treatment with the wild type. We examined 12 mutants in the oxidative stress pathway and the cell cycle pathway, in which 8 are found to be hypersensitive to plasma processing. The mutated genes’ roles in the two pathways are analyzed to understand the biological response mechanisms of plasma treatment. The results demonstrate that genes from both pathways are needed for the eukaryotic cells to survive the complex plasma treatment.