An SERS analytical protocol for characterizing native Japanese plant extracts
An SERS analytical protocol for characterizing native Japanese plant extracts
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用于表征日本本土植物提取物的 SERS 分析方案
DOI：
10.1002/jrs.5856
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发表时间：
2020
影响因子：
2.5
通讯作者：
et.al
中科院分区：
文献类型：
--
作者：
K. ITO;Brunetto G. Brunetti;et.al
鉴定古代纺织品中的天然染料对科学家来说是一个长期的挑战；然而，通过追溯传统的染色方法，并为子孙后代保护历史瑰宝，这项努力向染色大师的技能和知识致敬。本研究采用表面增强拉曼光谱法，对日本本土植物黄柏根、黄芩根、黄柏皮、黄柏皮、香柏干花芽和黄柏果的主要成分进行了测定。对粗提物和随后的再过滤和稀释提物的SERS检测数据进行分析合并，为联合鉴定萘醌（紫草素和烷烃）、生物碱（小檗碱）、多羟基黄酮（黄芩苷/黄芩素、杨梅素/杨梅三醇）、二芳基七烷类、烯丙基苯（丁香酚）和单宁（没食子酸/鞣花酸）提供了进一步有效和敏感的结果。将该分析方案的结果与更传统的色谱和质谱方法所获得的结果进行比较，提供并质疑有关普遍接受的标记成分和对映选择性判别的数据，这些数据有助于丰富着色剂生物来源数据库，用于确定历史上重要的日本着色剂和染料。
The identification of natural dyes in ancient textiles is a longstanding challenge for scientists; yet, the effort honours the skill and knowledge of master dyers by retracing traditional dyeing methods and assisting in conserving historical treasures for future generations. Here, the main constituents of selected native Japanese extracts from the roots ofLithospermum erythrorhizonandScutellaria baicalensis, the barks ofPhellodendron amurenseandMorella rubra, the dried flower bud ofSyzygium aromataticum, and the fruit ofTerminalia chebulaare determined by surface‐enhanced Raman spectroscopy following traditional Japanese extraction methods. The analytical amalgamation of data from SERS examinations on crude extracts and on subsequently refiltered and diluted extracts provides further effective and sensitive results for the combined identification of napthoquinones (shikonin and alkanin), alkaloid (berberine), polyhydroxyflavonoids (baicalin/baicalein, myricetin/myricitrin), diarylheptanoids, allylbenzene (eugenol), and tannins (gallic/ellagic acid). The comparison of results from this analytical protocol with those achieved by the more traditional chromatographic and mass spectrometric method provides and questions data regarding generally accepted marker components and enantioselective discriminations that serve to enrich the database of biological sources of colouring materials to be used in determining historically important Japanese colorants and dyestuffs.