Global mapping of herpesvirus-host protein complexes reveals a transcription strategy for late genes.
Global mapping of herpesvirus-host protein complexes reveals a transcription strategy for late genes.
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DOI：
10.1016/j.molcel.2014.11.026
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发表时间：
2015-01-22
期刊：
影响因子：
16
通讯作者：
Glaunsinger, Britt A.
中科院分区：
文献类型：
--
作者：
Davis, Zoe H.;Verschueren, Erik;Jang, Gwendolyn M.;Kleffman, Kevin;Johnson, Jeffrey R.;Park, Jimin;Von Dollen, John;Maher, M. Cyrus;Johnson, Tasha;Newton, William;Jaeger, Stefanie;Shales, Michael;Horner, Julie;Hernandez, Ryan D.;Krogan, Nevan J.;Glaunsinger, Britt A.
绘制宿主-病原体相互作用已被证明有助于了解病毒如何操纵宿主机制以及如何调节众多细胞过程。DNA病毒如疱疹病毒具有相对大的编码能力，因此可以靶向广泛的细胞蛋白质网络。为了鉴定被这种病原体劫持的宿主蛋白，我们系统地亲和标记并纯化了来自人类细胞的卡波西肉瘤相关疱疹病毒（KSHV）的所有89种蛋白。该物质的质谱鉴定出超过500种病毒-宿主相互作用。KSHV导致艾滋病相关癌症，其相互作用网络富含与癌症相关的蛋白质，并与HIV-1靶向的蛋白质重叠。我们发现保守的KSHV蛋白ORF 24结合RNA聚合酶II，并通过模拟和替换细胞TATA盒结合蛋白（TBP）将其带到病毒晚期启动子。这是疱疹病毒晚期基因表达所必需的，这是病毒生命周期的一个复杂且知之甚少的阶段。
Mapping host-pathogen interactions has proven instrumental for understanding how viruses manipulate host machinery and how numerous cellular processes are regulated. DNA viruses such as herpesviruses have relatively large coding capacity and thus can target an extensive network of cellular proteins. To identify the host proteins hijacked by this pathogen, we systematically affinity tagged and purified all 89 proteins of Kaposi’s sarcoma-associated herpesvirus (KSHV) from human cells. Mass spectrometry of this material identified over 500 virus-host interactions. KSHV causes AIDS-associated cancers and its interaction network is enriched for proteins linked to cancer and overlaps with proteins that are also targeted by HIV-1. We found that the conserved KSHV protein ORF24 binds to RNA polymerase II and brings it to viral late promoters by mimicking and replacing cellular TATA-box-binding protein (TBP). This is required for herpesviral late gene expression, a complex and poorly understood phase of the viral lifecycle.
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影响因子：
4.8
作者：
Jaeger, Stefanie;Gulbahce, Natali;Krogan, Nevan J.
通讯作者：
Krogan, Nevan J.
影响因子：
5.4
作者：
Jia, QM;Wu, TT;Sun, R
通讯作者：
Sun, R
影响因子：
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作者：
Navratil V;de Chassey B;Combe CR;Lotteau V
通讯作者：
Lotteau V
影响因子：
5.4
作者：
Arumugaswami, Vaithilingaraja;Wu, Ting-Ting;Sun, Ren
通讯作者：
Sun, Ren
影响因子：
6.7
作者：
Lee S;Salwinski L;Zhang C;Chu D;Sampankanpanich C;Reyes NA;Vangeloff A;Xing F;Li X;Wu TT;Sahasrabudhe S;Deng H;Lacount DJ;Sun R
通讯作者：
Sun R