Highly photoluminescent carbon dots-based immunosensors for ultrasensitive detection of aflatoxin M1 residues in milk
Highly photoluminescent carbon dots-based immunosensors for ultrasensitive detection of aflatoxin M1 residues in milk
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DOI：
10.1016/j.foodchem.2021.129443
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发表时间：
2021-03-31
期刊：
影响因子：
8.8
通讯作者：
Jiang, Wenxiao
中科院分区：
文献类型：
--
作者：
Li, Guangming;Liu, Chen;Jiang, Wenxiao
本文采用水热法合成了高发光的氮掺杂碳量子点，量子产率达到97.1%。基于碳量子点的内过滤效应，研制了一种免标记免疫传感器，用于牛奶中黄曲霉毒素M1残留的超灵敏检测。检测限为0.0186 ng/mL（相当于18.10 ng/kg），符合最严格的最大容许限度值25 ng/ kg。该免疫传感器在0.003 ~ 0.81 ng/mL范围内线性关系良好，牛奶样品加标回收率为79.6%~ 112.5%，相对标准偏差为6.7%~ 13.3%。与其他免疫检测方法相比，内滤效应免疫传感器将荧光检测技术与传统的酶级联扩增技术相结合，可作为一种可靠的黄曲霉毒素M1残留检测的现场筛选方法。
Here, a facile hydrothermal method was used to synthesize highly photoluminescent N-doped carbon dots, and the quantum yields reached 97.1%. Then, a label-free immunosensor based on the inner filter effect of carbon dots was developed for ultrasensitive detection of aflatoxin M1 residues in milk. The detection limit was 0.0186 ng/mL (equivalents to 18.10 ng/kg), which satisfied the most stringent maximum tolerable limit value of 25 ng/ kg. Besides, the immunosensor showed a good linear relationship from 0.003 ng/mL to 0.81 ng/mL, and the average recoveries ranged from 79.6% to 112.5% for spiked milk samples, with relative standard deviations ranging from 6.7% to 13.3%. Compared with other immunoassays, the inner filter effect-based immunosensor incorporating fluorescent detection into conventional enzymatic cascade amplification systems and could be a reliable on-site screening method for aflatoxin M1 residue analysis.