Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing
Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing
复制标题
使用碱基编辑产生小麦除草剂耐受性状和新的选择标记
DOI：
10.1038/s41477-019-0405-0
复制
发表时间：
2019-05-01
期刊：
影响因子：
18
通讯作者：
Gao, Caixia
中科院分区：
文献类型：
--
作者：
Zhang, Rui;Liu, Jinxing;Gao, Caixia
通过基因组编辑开发耐除草剂品种对于解决小麦种植中日益严重的杂草问题具有很大的希望。在这里，我们产生了无转基因小麦胚芽窝藏除草剂耐受性突变，赋予耐受性磺酰脲类，咪唑啉酮和芳氧基苯氧基丙酸类除草剂通过碱基编辑乙酰乳酸合成酶（ALS）和乙酰辅酶A羧化酶基因。这些可叠加的除草剂耐受性状为杂草管理提供了潜在的强大工具。此外，我们发现在小麦ALS Pro-174密码子（TaALS-P174）处的碱基编辑赋予小麦在MS生长培养基中对烟嘧磺隆除草剂的足够抗性以允许选择。当TaALS-P174编辑器与其他感兴趣的靶标的编辑器偶联时，在烟嘧磺隆抗性植物中发生共编辑，并且生长培养基中的抗性选择使偶联靶标的频率增加了几倍。这种可选择的协同编辑系统有可能极大地支持基础编辑在作物改良应用中的应用。
Developing herbicide-tolerant varieties by genome editing holds great promise for addressing the worsening weed problems in wheat cultivation. Here, we generated transgene-free wheat germplasms harbouring herbicide tolerance mutations that confer tolerance to sulfonylurea-, imidazolinone- and aryloxyphenoxy propionate-type herbicides by base editing the acetolactate synthase (ALS) and acetyl-coenzyme A carboxylase genes. These stackable herbicide tolerance traits provide a potentially powerful tool for weed management. In addition, we found that base editing at the wheat ALS Pro-174 codon (TaALS-P174) endowed wheat with sufficient resistance to nicosulfuron herbicide in MS growth medium to allow selection. When the TaALS-P174 editor was coupled with editors for other targets of interest, co-editing occurred in the nicosulfuron-resistant plants, and selection for resistance in growth medium enriched the frequency of coupled targets by several-fold. This selectable co-editing system has the potential to greatly bolster adoption of base editing for crop improvement applications.