Modulation of polyplex release from biodegradable microparticles through poly(ethylenimine) modification and varying loading concentration.
Modulation of polyplex release from biodegradable microparticles through poly(ethylenimine) modification and varying loading concentration.
复制标题
通过聚（乙烯亚胺）改性和改变负载浓度来调节可生物降解微粒中的复合物释放。
DOI：
10.1007/s11095-013-1133-1
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发表时间：
2014
影响因子：
3.7
通讯作者：
Mikos,AntoniosG
中科院分区：
文献类型：
--
作者：
Needham,ClarkJ;Shah,SaritaR;Mountziaris,PaschaliaM;Kasper,FKurtis;Mikos,AntoniosG
目的研究支化聚透明质酸（HA）的交联作用（乙烯亚胺）（bPEI）和这些聚合物与DNA复合的不同负载浓度对它们从聚乙烯亚胺（bPEI）中释放的影响。（DL-乳酸-羟基乙酸共聚物）（PLGA）微球和靶细胞的转染。方法为了检查改变基因递送聚合物对系统的影响，我们观察了形态、大小、负载效率，聚合物和DNA的释放，和转染效率的微粒形成与三个内部相加载浓度在microparticle formation.ResultsAddition的HA这个载体允许增加加载浓度在这些系统和显着改变释放动力学，而不改变粒子的形态。HA的bPEI骨干上的掺入显着增加了从相应的微粒formulation.ConclusionsThe结果表明，与HA和负载的聚合物/DNA复合物的浓度的bPEI的修饰释放的复合物的转染效率可以显着改变从PLGA微粒的截留和释放曲线。这是重要的，因为它提供了深入了解基因递送载体的修饰对控制释放系统的影响，该控制释放系统被设计为实现持续的治疗反应。
PurposeThis work investigates the effects of hyaluronic acid (HA) conjugated onto branched poly(ethylenimine) (bPEI) and varying loading concentrations of these polymers complexed with DNA on their release from poly(DL-lactic-co-glycolic acid) (PLGA) microparticles and the transfection of target cells.MethodsTo examine the effect of alteration of the gene delivery polymer on the system, we observed the morphology, size, loading efficiency, polymer and DNA release, and the transfection efficiency for the microparticles formed with three internal phase loading concentrations during microparticle formation.ResultsAddition of HA to this vector allowed for increased loading concentration within these systems and significantly altered release kinetics without changing the morphology of the particles. The incorporation of HA onto the bPEI backbone significantly increased the transfection efficiency of the complexes released from the corresponding microparticle formulation.ConclusionsThe results show that the modification of bPEI with HA and the concentration of loaded polymer/DNA complexes can significantly alter the entrapment and release profiles from PLGA microparticles. This is significant in that it offers insight into the effects of modification of gene delivery vectors on a controlled release system designed to achieve a sustained therapeutic response.