Evolutionary history of the Asr gene family
Evolutionary history of the Asr gene family
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DOI：
10.1016/j.gene.2006.05.010
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发表时间：
2006-08-15
期刊：
影响因子：
3.5
通讯作者：
Iusem, Norberto D.
中科院分区：
文献类型：
--
作者：
Frankel, Nicolas;Carrari, Fernando;Iusem, Norberto D.
Asr基因家族广泛存在于高等植物中。大多数Asr基因在不同的环境胁迫条件下和果实成熟过程中上调。ASR蛋白定位于细胞核中，其可能的功能是转录调节。在栽培番茄中，我们发现了一个新的第四个家族成员，命名为Asr 4，它的地图接近其兄弟基因Asr 1-Asr 2-Asr 3，并显示一个非共享区域编码的结构域包含一个13个氨基酸的重复。在这项工作中，我们能够扩大我们以前的分析Asr 2和调查的编码区的4个已知的Asr旁系同源基因在7个番茄品种来自不同的地理位置。此外，我们对ASR蛋白进行了系统发育分析。从这项工作中得出的第一个结论是，番茄ASR蛋白在树中聚集在一起。这种观察可以用协同进化或基因的诞生和死亡来解释。其次，我们的研究表明，Asr 1是高度保守的替代和同义位点内的番茄属。Asr 1蛋白序列保守性可能与其在不同组织中的多种功能有关，而低的同义替换率表明Asr 1的沉默变异受到选择性限制，这可能与其高表达水平有关。最后，我们发现，Asr 1激活水分胁迫下是不保守的番茄物种之间。(c)2006 Elsevier B. V.保留所有权利。
The Asr gene family is widespread in higher plants. Most Asr genes are up-regulated under different environmental stress conditions and during fruit ripening. ASR proteins are localized in the nucleus and their likely function is transcriptional regulation. In cultivated tomato, we identified a novel fourth family member, named Asr4, which maps close to its sibling genes Asr1-Asr2-Asr3 and displays an unshared region coding for a domain containing a 13-amino acid repeat. In this work we were able to expand our previous analysis for Asr2 and investigated the coding regions of the four known Asr paralogous genes in seven tomato species from different geographic locations. In addition, we performed a phylogenetic analysis on ASR proteins. The first conclusion drawn from this work is that tomato ASR proteins cluster together in the tree. This observation can be explained by a scenario of concerted evolution or birth and death of genes. Secondly, our study showed that Asr1 is highly conserved at both replacement and synonymous sites within the genus Lycopersicon. ASR1 protein sequence conservation might be associated with its multiple functions in different tissues while the low rate of synonymous substitutions suggests that silent variation in Asr1 is selectively constrained, which is probably related to its high expression levels. Finally, we found that Asr1 activation under water stress is not conserved between Lycopersicon species. (c) 2006 Elsevier B.V. All rights reserved.