Insecticidal juvenile hormone analogs stimulate the production of male offspring in the crustacean Daphnia magna
Insecticidal juvenile hormone analogs stimulate the production of male offspring in the crustacean Daphnia magna
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DOI：
10.1289/ehp.5982
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发表时间：
2003-06-01
影响因子：
10.4
通讯作者：
LeBlanc, GA
中科院分区：
文献类型：
--
作者：
Olmstead, AW;LeBlanc, GA
保幼激素类似物（JHA）代表一类专门设计用于破坏昆虫相对独特的内分泌调节过程的杀虫剂。最近我们证明了甲壳类juvenoid。激素甲基法呢酸程序卵母细胞的甲壳类动物大型蚤发展成为男性。我们假设，杀虫JHA可能模仿甲基法呢酸的行动，产生改变后代的性别比例。水蚤长期（3周）暴露于亚致死浓度的甲基法呢酸，JHA pyriproxyfen，和几个nonjuvenoid化学品，以辨别是否过量的雄性后代生产是一个通用的响应压力或特定的响应juvenoid激素。只有甲基法尼酸和吡丙醚增加了暴露的母体生物体产生的男性的百分比。如先前报道的与甲基法呢酸，急性暴露（24小时），无论是吡丙醚或JHA甲氧普烯造成卵母细胞成熟的卵巢发育成男性。我们进行了实验以确定JHA和法呢酸甲酯的组合效应是否更好地符合浓度相加模型（指示相同的作用机制）或独立的联合作用模型（指示不同的作用机制）。组合效果符合更好的浓度添加模型，虽然一些协同作用，未知的病因，杀虫剂和激素之间是显而易见的。这些实验表明，杀虫JHA模拟甲壳类保幼激素法呢酸甲酯的作用，导致雄性后代的不适当生产。在环境中发生这种影响可能对易受影响的甲壳类动物种群造成可怕的后果。
Juvenile hormone analogs (JHAs) represent a class of insecticides that were designed specifically to disrupt endocrine-regulated processes relatively unique to insects. Recently we demonstrated that the crustacean juvenoid. hormone methyl farnesoate programs oocytes of the crustacean Daphnia magna to develop into males. We hypothesized that insecticidal JHAs might mimic the action of methyl farnesoate, producing altered sex ratios of offspring. Daphnids were exposed chronically (3 weeks) to sublethal concentrations of methyl farnesoate, the JHA pyriproxyfen, and several nonjuvenoid chemicals to discern whether excess male offspring production is a generic response to stress or a specific response to juvenoid hormones. Only methyl Farnesoate and pyriproxyfen increased the percentage of males produced by exposed maternal organisms. As previously reported with methyl farnesoate, acute exposure (24 hr) to either pyriproxyfen or the JHA methoprene caused oocytes maturing in the ovary to develop into males. We performed experiments to determine whether combined effects of a JHA and methyl farnesoate conformed better to a model of concentration addition (indicative of same mechanism of action) or independent joint action (indicative of different mechanisms of action). Combined effects conformed better to the concentration-addition model, although some synergy, of unknown etiology, was evident between the insecticides and the hormone. These experiments demonstrate that insecticidal JHAs mimic the action of the crustacean juvenoid hormone methyl farnesoate, resulting in the inappropriate production of male offspring. The occurrence of such an effect in the environment could have dire consequences on susceptible crustacean populations.