Red-Green-Blue Electrogenerated Chemiluminescence Utilizing a Digital Camera as Detector
Red-Green-Blue Electrogenerated Chemiluminescence Utilizing a Digital Camera as Detector
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DOI：
10.1021/ac404135f
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发表时间：
2014-03-04
影响因子：
7.4
通讯作者：
Francis, Paul S.
中科院分区：
文献类型：
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作者：
Doeven, Egan H.;Barbante, Gregory J.;Francis, Paul S.
利用伴随电化学发光团的独特激发和发射特性以及传统数码相机固有的颜色选择性，我们创建了一种用于多重电化学发光检测的新策略，适合开发低成本、便携式临床诊断设备。红色、绿色和蓝色发射器可以在 ECL 强度与施加电势和发射波长的三维空间内有效解析。由于每个发射器对摄影 RGB 通道的相对贡献比是恒定的，因此 RGB ECL 强度与施加电势的曲线可以有效地隔离到每个电势下的单个发射器。
Exploiting the distinct excitation and emission properties of concomitant electrochemiluminophores in conjunction with the inherent color selectivity of a conventional digital camera, we create a new strategy for multiplexed electrogenerated chemiluminescence detection, suitable for the development of low-cost, portable clinical diagnostic devices. Red, green and blue emitters can be efficiently resolved over the three-dimensional space of ECL intensity versus applied potential and emission wavelength. As the relative contribution ratio of each emitter to the photographic RGB channels is constant, the RGB ECL intensity versus applied-potential curves could be effectively isolated to a single emitter at each potential.