Mechanism of Arachidonic Acid Accumulation during Aging in Mortierella alpina: A Large-Scale Label-Free Comparative Proteomics Study
Mechanism of Arachidonic Acid Accumulation during Aging in Mortierella alpina: A Large-Scale Label-Free Comparative Proteomics Study
复制标题
高山被孢霉衰老过程中花生四烯酸积累的机制：大规模无标记比较蛋白质组学研究
DOI：
10.1021/acs.jafc.6b03284
复制
发表时间：
2016
影响因子：
6.1
通讯作者：
Huang He
中科院分区：
文献类型：
--
作者：
Yu Yadong;Li Tao;Wu Na;Ren Lujing;Jiang Ling;Ji Xiaojun;Huang He
花生四烯酸（ARA）是一种重要的多不饱和脂肪酸，对人体具有多种有益的生理作用。长期以来，人们一直认为高山被孢霉的老化可以显著提高ARA的产量，但其确切的机制仍不清楚。本文采用大规模无标记比较蛋白质组学等方法对上述机制进行了系统研究。超微结构观察发现，异常线粒体聚集在萎缩的脂滴周围。蛋白质组学分析显示，共有171个蛋白质在衰老过程中的表达发生了显着变化。途径分析表明，活性氧（ROS）的积累和刺激激活的苹果酸/丙酮酸循环和异柠檬酸脱氢酶，这可能提供额外的NADPH合成ARA。EC 4.2.1.17-水合酶可能是衰老过程中ARA积累的关键因素。这些研究结果为进一步提高衰老M. alpina产生的油中ARA含量的努力提供了宝贵的资源。
Arachidonic acid (ARA) is an important polyunsaturated fatty acid having various beneficial physiological effects on the human body. The aging ofMortierella alpinahas long been known to significantly improve ARA yield, but the exact mechanism is still elusive. Herein, multiple approaches including large-scale label-free comparative proteomics were employed to systematically investigate the mechanism mentioned above. Upon ultrastructural observation, abnormal mitochondria were found to aggregate around shrunken lipid droplets. Proteomics analysis revealed a total of 171 proteins with significant alterations of expression during aging. Pathway analysis suggested that reactive oxygen species (ROS) were accumulated and stimulated the activation of the malate/pyruvate cycle and isocitrate dehydrogenase, which might provide additional NADPH for ARA synthesis. EC 4.2.1.17-hydratase might be a key player in ARA accumulation during aging. These findings provide a valuable resource for efforts to further improve the ARA content in the oil produced by agingM. alpina.