Arabidopsis Chromatin Remodeling Factor PICKLE Interacts with Transcription Factor HY5 to Regulate Hypocotyl Cell Elongation
Arabidopsis Chromatin Remodeling Factor PICKLE Interacts with Transcription Factor HY5 to Regulate Hypocotyl Cell Elongation
复制标题
拟南芥染色质重塑因子 PICKLE 与转录因子 HY5 相互作用调节下胚轴细胞伸长
DOI：
10.1105/tpc.112.105742
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发表时间：
2013-01-01
期刊：
影响因子：
11.6
通讯作者：
Lin, Rongcheng
中科院分区：
文献类型：
--
作者：
Jing, Yanjun;Zhang, Dong;Lin, Rongcheng
光形态建成是一个重要的植物发育过程，涉及光介导的转录组变化、组蛋白修饰和下胚轴生长抑制。然而，基于染色质的调控机制在这一过程中仍然在很大程度上是未知的。在这里，我们确定增强的光形态发生1（EPP 1），以前被称为PICKLE（PKL），一个ATP依赖的染色质重塑因子的chromodomain/解旋酶/DNA结合家族，作为一个阻遏物的光形态发生在拟南芥。我们发现PKL/EPP 1的表达在下胚轴中以光感受器依赖的方式被光抑制。此外，我们揭示了转录因子ELONGATED HYPOCOTYL 5（HY 5）结合到细胞伸长相关基因的启动子上，并通过它们的物理相互作用招募PKL/EPP 1。PKL/EPP 1反过来通过抑制靶位点组蛋白H3 Lys 27的三甲基化来负调控HY 5，从而调控这些基因的表达，从而调控下胚轴伸长。我们还表明，HY 5具有转录抑制活性。我们的研究揭示了染色质重塑因子在抑制光形态建成中的关键作用，并表明转录因子介导的染色质重塑机制的招募对于植物发育响应光环境变化至关重要。
Photomorphogenesis is a critical plant developmental process that involves light-mediated transcriptome changes, histone modifications, and inhibition of hypocotyl growth. However, the chromatin-based regulatory mechanism underlying this process remains largely unknown. Here, we identify ENHANCED PHOTOMORPHOGENIC1 (EPP1), previously known as PICKLE (PKL), an ATP-dependent chromatin remodeling factor of the chromodomain/helicase/DNA binding family, as a repressor of photomorphogenesis in Arabidopsis thaliana. We show that PKL/EPP1 expression is repressed by light in the hypocotyls in a photoreceptor-dependentmanner. Furthermore, we reveal that the transcription factor ELONGATED HYPOCOTYL5 (HY5) binds to the promoters of cell elongation-related genes and recruits PKL/EPP1 through their physical interaction. PKL/EPP1 in turn negatively regulates HY5 by repressing trimethylation of histone H3 Lys 27 at the target loci, thereby regulating the expression of these genes and, thus, hypocotyl elongation. We also show that HY5 possesses transcriptional repression activity. Our study reveals a crucial role for a chromatin remodeling factor in repressing photomorphogenesis and demonstrates that transcription factor-mediated recruitment of chromatin-remodeling machinery is important for plant development in response to changing light environments.