Cell lineage, cell-cell interaction, and segment formation in the ectoderm of a glossiphoniid leech embryo.
Cell lineage, cell-cell interaction, and segment formation in the ectoderm of a glossiphoniid leech embryo.
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DOI：
10.1016/0012-1606(84)90044-7
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发表时间：
1984-07
影响因子：
2.7
通讯作者：
Saul L. Zackson
中科院分区：
文献类型：
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作者：
Saul L. Zackson
本文用细胞系示踪染料研究了舌管蛭Helobdella triserialis芽带的细胞分裂方式和细胞间相互作用。Helobella胚胎的每个胚带由5个原始胚细胞柱或带组成，称为中胚层m带和外胚层n、o、p和q带。每个外胚层带的初级原始细胞似乎经历定型的，谱系特异性的细胞分裂。因此，水蛭的同色异谱分裂模式似乎是通过一系列同源原代母细胞克隆的一系列分段迭代、定型的细胞分裂而产生的。通过细胞消融研究细胞-细胞相互作用。除了一个例外，原始细胞在进行常规分裂时，并不考虑它们正常邻居的存在与否。在一个例外的情况下，o原始细胞进行分裂正常特征的p原始细胞时，其正常的相邻p带被删除。然而，当相邻的m、n和q带被删除时，o和p母细胞都进行了正常的定型分裂。有人建议，由o和p原始细胞的通路的差异选择取决于它们的相对位置相对于彼此和外部的germinal带的极性线索。
Cell division patterns and cell-cell interactions in the germinal bands of the glossiphoniid leechHelobdella triserialiswere studied with the aid of a cell lineage tracer dye. Each germinal band of theHelobdellaembryo consists of five columns, orbandlets, of primary blast cells, designated as the mesodermal m bandlet and ectodermal n, o, p, and q bandlets. Primary blast cells of each ectodermal bandlet appear to undergo stereotyped, lineage-specific cell divisions. The metameric segmentation pattern of the leech thus appears to arise through a series of segmentally iterated, stereotyped cell divisions of serially homologous primary blast cell clones. Cell-cell interactions were studied by means of cell ablations. With one exception, blast cells underwent their stereotyped divisions without regard to the presence or absence of their normal neighbors. In the one exceptional case, o blast cells underwent divisions normally characteristic of p blast cells when their normal neighboring p bandlet was deleted. However, both o and p blast cells underwent their normal stereotyped divisions when their neighboring m, n, and q bandlets were deleted. It is proposed that the differential choice of pathway by the o and p blast cells depends upon their relative position with respect to each other and to a polarity cue external to the germinal band.