Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli
Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli
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DOI：
10.1128/aac.01116-06
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发表时间：
2007-02-01
影响因子：
4.9
通讯作者：
Champney, W. Scott
中科院分区：
文献类型：
--
作者：
McGaha, Susan M.;Champney, W. Scott
在大肠杆菌细胞中检测了氨基糖苷类抗生素潮霉素B对翻译和核糖体亚基形成的抑制作用。进行脉冲追踪标记实验，其验证了药物处理的细胞中核糖体亚基合成的较低速率。潮霉素B对活细胞数、生长速率、蛋白质合成以及30 S和SOS亚基形成具有浓度依赖性抑制作用。与其他氨基糖苷类抗生素不同，潮霉素B对E.杆菌从处理的细胞的总RNA的检查表明，相应的16S rRNA的前体的RNA的增加，而成熟的16S rRNA减少。经潮霉素B处理的细胞中的rRNA的北方杂交表明，RNase II和RNase III缺陷的E.大肠杆菌在药物处理后积累了16S rRNA片段。结果表明潮霉素B靶向蛋白质合成和30 S核糖体亚基组装。
The aminoglycoside antibiotic hygromycin B was examined in Escherichia coli cells for inhibitory effects on translation and ribosomal-subunit formation. Pulse-chase labeling experiments were performed, which verified lower rates of ribosomal-subunit synthesis in drug-treated cells. Hygromycin B exhibited a concentration-dependent inhibitory effect on viable-cell numbers, growth rate, protein synthesis, and 30S and SOS subunit formation. Unlike other aminoglycosides, hygromycin B was a more effective inhibitor of translation than of ribosomal-subunit formation in E. coli. Examination of total RNA from treated cells showed an increase in RNA corresponding to a precursor to the 16S rRNA, while mature 16S rRNA decreased. Northern hybridization to rRNA in cells treated with hygromycin B showed that RNase II- and RNase III-deficient strains of E. coli accumulated 16S rRNA fragments upon treatment with the drug. The results indicate that hygromycin B targets protein synthesis and 30S ribosomal-subunit assembly.