A PLUS-END-DIRECTED MOTOR ENZYME THAT MOVES ANTIPARALLEL MICROTUBULES INVITRO LOCALIZES TO THE INTERZONE OF MITOTIC SPINDLES
A PLUS-END-DIRECTED MOTOR ENZYME THAT MOVES ANTIPARALLEL MICROTUBULES INVITRO LOCALIZES TO THE INTERZONE OF MITOTIC SPINDLES
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DOI：
10.1038/359543a0
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发表时间：
1992-10-08
期刊：
影响因子：
64.8
通讯作者：
MCINTOSH, JR
中科院分区：
文献类型：
--
作者：
NISLOW, C;LOMBILLO, VA;MCINTOSH, JR
有丝分裂包括一组复杂的重叠运动事件，其中许多涉及微管依赖的运动酶1，2。在这里，我们描述了Kinesin超家族的一个新成员。该蛋白最初被CHO1单抗3鉴定为纺锤体抗原，并被证明是有丝分裂过程所必需的4，5。我们克隆了编码该抗原的基因，发现其序列包含一个与运动素样蛋白的运动区具有很强序列相似性的结构域。该基因的产物在细菌中表达，可以在体外跨越反平行微管，在镁-三磷酸腺苷存在的情况下，微管以一种让人想起纺锤体延长过程中微管运动的方式相互滑动。
MITOSIS comprises a complex set of overlapping motile events, many of which involve microtubule-dependent motor enzymes1,2. Here we describe a new member of the kinesin superfamily. The protein was originally identified as a spindle antigen by the CHO1 monoclonal antibody3 and shown to be required for mitotic progression4,5. We have cloned the gene that encodes this antigen and found that its sequence contains a domain with strong sequence similarity to the motor domain of kinesin-like proteins. The product of this gene, expressed in bacteria, can cross-bridge antiparallel microtubules in vitro, and in the presence of Mg-ATP, microtubules slide over one another in a fashion reminiscent of microtubule movements during spindle elongation.