The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability
The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability
复制标题
DOI：
10.1016/s0092-8674(01)00504-9
复制
发表时间：
2001-10-05
期刊：
影响因子：
64.5
通讯作者：
Greider, CW
中科院分区：
文献类型：
--
作者：
Hemann, MT;Strong, MA;Greider, CW
端粒功能的丧失可诱导细胞周期停滞和凋亡。为了研究触发细胞对端粒功能障碍的反应的过程，我们将具有短端粒的mTR-/- G6小鼠与具有长端粒的端粒酶杂合子（mTR+/-）小鼠杂交。端粒酶缺失的后代的表型与晚代亲本的表型相似，尽管只有一半的染色体是短的。引人注目的是，光谱[核型分析（SKY）]显示端粒功能的丧失优先发生在端粒短的染色体上。我们的数据表明，虽然在大多数研究中测量平均端粒长度，但在没有端粒酶的情况下，构成端粒功能障碍并限制细胞存活的不是平均端粒，而是最短的端粒。
Loss of telomere function can induce cell cycle arrest and apoptosis. To investigate the processes that trigger cellular responses to telomere dysfunction, we crossed mTR-/- G6 mice that have short telomeres with mice heterozygous for telomerase (mTR+/-) that have long telomeres. The phenotype of the telomerase null offspring was similar to that of the late generation parent, although only half of the chromosomes were short. Strikingly, spectra[ karyotyping (SKY) analysis revealed that loss of telomere function occurred preferentially on chromosomes with critically short telomeres. Our data indicate that, while average telomere length is measured in most studies, it is not the average but rather the shortest telomeres that constitute telomere dysfunction and limit cellular survival in the absence of telomerase.