Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar.
Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar.
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DOI：
10.1007/s007840050126
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发表时间：
2000-06-01
影响因子：
3.4
通讯作者：
Goldberg, M
中科院分区：
文献类型：
--
作者：
Decup, F;Six, N;Goldberg, M
骨涎蛋白（BSP）是一种成骨蛋白（OP），与载体混合后植入大鼠第一上磨牙髓内（OP组）。用牙科布尔斯钻制备洞，用钢探针尖端加压进行牙髓穿孔。在8、14和30天后，处死大鼠，并将OP组的牙髓与（1）假手术组（S组）、（2）单独植入载体的组（C组）和（3）用氢氧化钙覆盖的组（Ca组）进行比较。8天后，可见少量炎性细胞，主要位于穿孔附近的牙髓表面。在钙组中，牙本质桥开始形成，与其他组相反。15天后，S和C组的牙髓中可见球形结构。在Ca组中，修复性骨牙本质桥将牙髓与腔体隔离。在OP组中观察到不同的反应，有一些细胞和基质排列或骨牙本质栓与修复性牙本质内层连续的证据。30天后，在S和C组的牙髓中观察到不规则的骨牙本质形成，具有球形结构合并的趋势，但球间间隙被牙髓残留物填充。Ca组髓室近中见骨牙本质。在BSP植入组中，成骨蛋白刺激形成均匀的牙本质样存款占据了大部分的近中部分的牙髓。显然，BSP刺激细胞的分化，这些细胞分泌有组织的细胞外基质，比迄今为止使用的任何其他加帽材料更有效。总之，本文报道的结果支持骨唾液酸蛋白显示新的生物活性特性，并且能够在1个月的时间内刺激牙髓中厚的修复性牙本质组织的发育，闭塞穿孔并填充近中三分之一的牙髓腔。
Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the pulp of rat first upper molars (OP group). Cavities were prepared with dental burs and pulp perforation was carried out by pressure with the tip of a steel probe. After 8, 14, and 30 days, the rats were killed and the pulps of the OP group were compared with (1) a sham group (S group), (2) a group where the carrier was implanted alone (C group), and (3) capping with calcium hydroxide (Ca group). After 8 days, a few inflammatory cells were seen, mostly located at the pulp surface near the perforation. In the Ca group, a dentin bridge started to form, in contrast to the other groups. After 15 days, globular structures were seen in the pulps of the S and C groups. A reparative osteodentin bridge isolated the pulp from the cavity in the Ca group. Variable reactions were seen in the OP group, with some evidence of cell and matrix alignments or plugs of osteodentin in continuity with an inner layer of reparative dentin. After 30 days, irregular osteodentin formation was observed in the pulps of the S and C groups, with a tendency for globular structures to merge, but with interglobular spaces filled by pulp remnants. In the Ca group, osteodentin was observed in the mesial part of the pulp chamber. In the BSP-implanted group, the osteogenic protein stimulated the formation of a homogeneous dentin-like deposit occupying most of the mesial part of the pulp. Apparently, BSP stimulates the differentiation of cells which secrete an organized extracellular matrix more efficiently than any other capping material used so far. Altogether, the results reported here support that bone sialoprotein displays novel bioactive properties and is capable of stimulating in 1 month's time the development of a thick reparative dentinal tissue in the pulp, occluding the perforation and filling the mesial third of the pulp chamber.