Concentration of aerosolized 99mTc-albumin in the pulmonary lymph of anesthetized sheep.
Concentration of aerosolized 99mTc-albumin in the pulmonary lymph of anesthetized sheep.
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麻醉绵羊肺淋巴液中雾化 99mTc-白蛋白的浓度。
DOI：
10.1152/jappl.1990.68.3.1233
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Dickerson,KD
中科院分区：
文献类型：
--
作者：
Peterson,BT;Dickerson,KD
我们研究了淋巴浓度的99 mTc-白蛋白沉积在麻醉绵羊的空气空间，以确定是否反映肺上皮功能的变化浓度的变化。5只对照羊用99 mTc-白蛋白气雾剂通气6 min，并在接下来的2 h内监测肺淋巴示踪剂的浓度。在最后45分钟内，淋巴浓度稳定在空气空间中估计值的0.03 +/- 0.01%。在7只绵羊中，通过将左心房压力增加20 cmH 2 O持续4小时诱导肺血管性高血压，使肺淋巴流量从基线值3 +/- 2增加到21 +/- 14 ml/h。这导致淋巴液中99 mTc-白蛋白浓度加倍至空气空间浓度的0.07 +/- 0.03%（P <0.01）。在另外7只绵羊中，静脉输注0.08-0.10 ml/kg油酸引起的肺损伤使99 mTc-白蛋白的淋巴浓度增加10倍，达到空气空间浓度的0.31 ± 0.09%（P <0.01）。肺血管高压绵羊体内示踪剂浓度的增加可能是淋巴流量增加导致示踪剂转移到淋巴管的结果。然而，数学模型表明，在肺损伤的绵羊中淋巴浓度增加10倍主要是由于上皮细胞的渗透性和表面积增加，上皮细胞参与了99 mTc-白蛋白从空气空间转移到肺组织中，并通过呼吸系统排出。（250字处删节）
We examined the lymphatic concentration of 99mTc-albumin deposited in the air spaces of anesthetized sheep to determine whether changes in the concentration reflected changes in lung epithelial function. Five control sheep were ventilated with an aerosol of 99mTc-albumin for 6 min, and the lung lymphatic concentration of the tracer was monitored for the next 2 h. During the last 45 min the lymphatic concentration stabilized at a value that was 0.03 +/- 0.01% of the estimated value in the air spaces. Pulmonary vascular hypertension, induced in seven sheep by increasing the left atrial pressure 20 cmH2O for 4 h, increased the lung lymph flow from a base-line value of 3 +/- 2 to 21 +/- 14 ml/h. This caused the concentration of the 99mTc-albumin in the lymph to double to 0.07 +/- 0.03% of the air space concentration (P less than 0.01). Lung injury induced by infusing 0.08-0.10 ml/kg oleic acid intravenously in seven other sheep increased the lymphatic concentration of the 99mTc-albumin 10-fold to 0.31 +/- 0.09% of the air space concentration (P less than 0.01). The increased tracer concentration in the sheep with pulmonary vascular hypertension could be the result of the increased lymph flow causing a diversion of tracer into the lymphatics. However, a mathematical model showed that the 10-fold increase in the lymphatic concentration in the sheep with lung injury was primarily the result of an increase in both permeability and surface area of the epithelium that participated in the transfer of the 99mTc-albumin from the air spaces into the lung tissue drained by the lymphatics.(ABSTRACT TRUNCATED AT 250 WORDS)