Mid-infrared (MIR) metabolic fingerprinting of amniotic fluid: A possible avenue for early diagnosis of prenatal disorders?
Mid-infrared (MIR) metabolic fingerprinting of amniotic fluid: A possible avenue for early diagnosis of prenatal disorders?
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DOI：
10.1016/j.aca.2012.12.023
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发表时间：
2013-02-18
影响因子：
6.2
通讯作者：
Gil, Ana M.
中科院分区：
文献类型：
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作者：
Graca, Goncalo;Moreira, Ana Sofia;Gil, Ana M.
本研究对妊娠中期羊水进行了中红外(MIR)代谢谱研究，研究对象为胎儿畸形(FM)、早产(PTD)和胎膜早破(PROM)，后两种情况发生在妊娠后期。在蒙特卡罗交叉验证(MCCV)的支持下，获得了FM和PTD前受试者组的偏最小二乘判别分析(PLS-DA)模型，并识别了特定的MIR谱变化。对于毕业舞会前的受试者，注意到了微小的变化。MIR的解释由内部(MIR/MIR)和(MIR/核磁共振)区域间的统计相关分析辅助，结果揭示了FM和PTD前受试者可能的生物标记物MIR特征。生物流体MIR代谢图谱作为一种低成本、易用、快速的方法具有诱人的可能性，目前的结果表明，它对临床诊断的疾病，如FM和PTD的临床前阶段具有敏感性。讨论了具体的改进需求，即关于样本数和实验重复性。(C)2012爱思唯尔B.V.保留所有权利。
This work describes a mid-infrared (MIR) metabolic profiling study of 2nd trimester amniotic fluid in relation to selected prenatal disorders, with results focusing on fetal malformations (FM), preterm delivery (PTD) and premature rupture of membranes (PROM), the latter two conditions occurring later in pregnancy. Partial least squares-discriminant analysis (PLS-DA) models were obtained for FM and pre-PTD subject groups, supported by Monte Carlo Cross Validation (MCCV), and identified specific MIR profile changes. For pre-PROM subjects, minor changes were noted. MIR interpretation was assisted by intra(MIR/MIR) and inter- (MIR/NMR) domain statistical correlation analysis, the results unveiling possible biomarker MIR signatures for FM and pre-PTD subjects. Biofluid MIR metabolic profiling holds enticing possibilities as a low cost, easy to use, rapid method and the results presented have shown its sensitivity to clinically diagnosed conditions such as FM, and to the pre-clinical stages of PTD. Specific improvement needs are discussed, namely regarding sample numbers and experimental reproducibility. (C) 2012 Elsevier B.V. All rights reserved.