Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles
Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles
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DOI：
10.1007/s10126-018-9825-5
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发表时间：
2018-04
影响因子：
3
通讯作者：
Keisuke Motone;T. Takagi;Shunsuke Aburaya;Wataru Aoki;Natsuko Miura;H. Minakuchi;H. Takeyama;Y. Nagasaki;Chuya Shinzato;M. Ueda
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作者：
Keisuke Motone;T. Takagi;Shunsuke Aburaya;Wataru Aoki;Natsuko Miura;H. Minakuchi;H. Takeyama;Y. Nagasaki;Chuya Shinzato;M. Ueda
珊瑚礁是地球上最具生物多样性和经济重要性的生态系统之一。然而，由于全球变暖导致海水温度上升，全世界都有珊瑚礁遭到破坏的报告。在这项研究中，我们研究了氧化还原纳米颗粒（RNPO）抑制活性氧（ROS）的潜力，活性氧在热应激下过度产生，并在导致珊瑚死亡中起着至关重要的作用。当造礁珊瑚（Acropora tenuis）幼虫在没有藻类共生体的情况下暴露于33 °C的热应力时，RNPO处理显著提高了存活率。首次采用纳米液相色谱-串联质谱技术对珊瑚幼虫进行蛋白质组分析。结果表明，在A. RNPO处理的幼鱼体内不存在这些蛋白质，表明RNPO能有效地清除A.薄壳幼虫从这项研究的结果表明，RNPO处理可以减少活性氧在离共生珊瑚幼虫，将是一个很有前途的方法，保护珊瑚从热应力。
Coral reefs are one of the most biologically diverse and economically important ecosystems on earth. However, the destruction of coral reefs has been reported worldwide owing to rising seawater temperature associated with global warming. In this study, we investigated the potential of a redox nanoparticle (RNPO) to scavenge reactive oxygen species (ROS), which are overproduced under heat stress and play a crucial role in causing coral mortality. When reef-building coral (Acropora tenuis) larvae, without algal symbionts, were exposed to thermal stress at 33 °C, RNPOtreatment significantly increased the survival rate. Proteome analysis of coral larvae was performed using nano-liquid chromatography-tandem mass spectrometry for the first time. The results revealed that several proteins related to ROS-induced oxidative stress were specifically identified inA. tenuislarvae without RNPOtreatment, whereas these proteins were absent in RNPO-treated larvae, which suggested that RNPOeffectively scavenged ROS fromA. tenuislarvae. Results from this study indicate that RNPOtreatment can reduce ROS in aposymbiotic coral larvae and would be a promising approach for protecting corals from thermal stress.