Prognostic significance of the MUC1 and MUC4 expressions in thyroid papillary carcinoma
Prognostic significance of the MUC1 and MUC4 expressions in thyroid papillary carcinoma
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DOI：
10.1097/mlg.0b013e31803d1720
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发表时间：
2007-05-01
期刊：
影响因子：
2.6
通讯作者：
Jung, Kwang-Yoon
中科院分区：
文献类型：
--
作者：
Baek, Seung-Kuk;Woo, Jeong-Soo;Jung, Kwang-Yoon
目标.本研究的目的是检测粘蛋白基因MUC1和MUC4在正常甲状腺组织、滤泡性腺瘤和乳头状癌中的表达，并探讨其表达差异。此外，我们的目的是估计其在乳头状癌的预后意义。方法.我们进行了半定量逆转录聚合酶链反应，以确定MUC1和MUC4 mRNA的表达，并进行免疫组化染色，以确定MUC1和MUC4蛋白表达在22例正常甲状腺组织，22例滤泡性腺瘤，15例乳头状癌。对87例甲状腺乳头状癌患者进行免疫组化半定量评分，并与预后因素进行比较，以评价其预后意义。结果：乳头状癌组织中MUC1mRNA的表达水平明显高于其他组织。而MUC4 mRNA在所有标本中均呈弱表达，且三组间无显著性差异。MUC1免疫反应在乳头状癌中更强，但在其他组织中不强。MUC4在所有甲状腺组织中均不表达。MUC1的表达与肿瘤大小、甲状腺外浸润和TNM分期有关（P
Objectives. The aim of the present study was to examine the expressions of mucin genes MUC1 and MUC4 and to evaluate the difference of their expressions in normal thyroid tissue, follicular adenoma, and papillary carcinoma. Furthermore, we aimed to estimate their prognostic significance in papillary carcinoma. Methods. We performed semiquantitative reverse-transcription polymerase chain reaction for determining the MUC1 and MUC4 mRNA expressions, and immunohistochemical staining was performed to determine the MUC1 and MUC4 protein expressions in 22 normal thyroid tissues, 22 follicular adenomas, and 15 papillary carcinomas. The semiquantitative scoring of the immunohistochemical staining was compared with the prognostic factors for thyroid carcinoma to evaluate the prognostic significance in 87 papillary carcinoma patients. Results: The MUC1 mRNA of the papillary carcinoma tissue showed an increased expression level compared with the other tissues. However, the expression level of MUC4 mRNA was weak in all the specimens, and this was not significantly different among the three groups. MUC1 immunoreactivity was more intense in papillary carcinoma but not in the other tissues. MUC4 was not expressed in all thyroid tissues. The expression of MUC1 was significantly associated with tumor size, extrathyroidal spread, and TNM stage (P