METABOLISM OF ZEARALENONE BY SOW INTESTINAL-MUCOSA INVITRO
METABOLISM OF ZEARALENONE BY SOW INTESTINAL-MUCOSA INVITRO
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DOI：
10.1016/0278-6915(87)90101-3
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发表时间：
1987-09-01
影响因子：
4.3
通讯作者：
KIESSLING, KH
中科院分区：
文献类型：
--
作者：
OLSEN, M;PETTERSSON, H;KIESSLING, KH
在NADPH或UDPGA存在下，将来自母猪的均质化肠粘膜样品与玉米赤霉烯酮孵育。此外，使用1-萘酚作为底物测定微粒体组分粘膜中的UDP葡萄糖醛酸基转移酶活性。在NADPH存在下，玉米赤霉烯酮被还原成α-谷氨酰胺和α-谷氨酰胺。和β-玉米赤霉烯醇（0.37 ± 0.01）; 0.18和0.29 ±。0.11在十二指肠和空肠中分别为nmol减少/mg蛋白/hr）。β-异构体是主要代谢物。玉米赤霉烯酮的葡糖苷酸结合与发生的还原水平相比非常高（11.3 . ±. 6.1和9.4 .+-。5.8在十二指肠和空肠中分别为nmol缀合物/ml蛋白/hr）。玉米赤霉烯酮和1-萘酚的葡糖苷酸结合率之间没有相关性，表明它们取决于UDP葡糖苷酸转移酶的两种不同同工酶。
Homogenized intestinal mucosa samples from sows were incubated with zearalenone in the presence of NADPH or UDPGA. In addition, UDPglucuronosyltransferase activity in the microsomal fraction mucosa was determined using 1-naphthol as substrate. In the presence of NADPH, zearalenone was reduced to both .alpha.- and .beta.-zearalenol (0.37 .+-. 0.18 and 0.29 .+-. 0.11 nmol reduced/mg protein/hr in the duodenum and jejunum, respectively). The .beta.-isomer was the predominant metabolite. Glucuronide conjugation of zearalenone was very high compared with the level of reduction occurring (11.3 .+-. 6.1 and 9.4 .+-. 5.8 nmol conjugated/ml protein/hr in the duodenum and jejunum, respectively). There was no correlation between the rates of glucuronide conjugation of zearalenone and 1-naphthol, indicating that they depend upon two different isoenzymes of UDPglucuronosyltransferase.