Functional conservation between yeast and plant endosomal Na+/H+ antiporters
Functional conservation between yeast and plant endosomal Na+/H+ antiporters
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DOI：
10.1016/s0014-5793(00)01412-5
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发表时间：
2000-04-14
期刊：
影响因子：
3.5
通讯作者：
Pardo, JM
中科院分区：
文献类型：
--
作者：
Quintero, FJ;Blatt, MR;Pardo, JM
Na+的胞内区室化是耐盐性的重要机制，因为它降低细胞溶质Na+水平，同时有助于细胞膨压和扩张的渗透调节。拟南芥的AtNHX 1蛋白在功能上取代了酵母的内体Na+/H+反向转运蛋白ScNHX 1，AtNHX 1和ScSHX 1赋予的离子耐受性与离子摄取到细胞内池中相关，该池在能量上依赖于液泡（H+）ATP酶。AtNX 1定位于酵母的液泡膜组分。因此，这两种转运蛋白在Na+区室化中具有进化上保守的功能，AtNHX 1 mRNA水平在叶片中被阿坝和NaCl处理上调，但在根组织中不上调。(C)2000年欧洲生物化学学会联合会。
Vacuolar compartmentation of Na+ is an essential mechanism for salinity tolerance since it lowers cytosolic Na+ levels while contributing to osmotic adjustment for cell turgor and expansion, The AtNHX1 protein of,Arabidopsis thaliana substituted functionally for ScNHX1, the endosomal Na+/H+ antiporter of yeast, Ion tolerance conferred by AtNHX1 and ScSHX1 correlated with ion uptake into an intracellular pool that aas energetically dependent on the vacuolar (H+)ATPase. AtNX1 localized to vacuolar membrane fractions of yeast. Hence, both transporters share an evolutionarily conserved function in Na+ compartmentation, AtNHX1 mRNA levels were upregulated by ABA and NaCl treatment in leaf but not in root tissue. (C) 2000 Federation of European Biochemical Societies.