Compatibility and entry exclusion of IncA and IncC plasmids revisited: IncA and IncC plasmids are compatible
Compatibility and entry exclusion of IncA and IncC plasmids revisited: IncA and IncC plasmids are compatible
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DOI：
10.1016/j.plasmid.2018.02.002
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发表时间：
2018-03-01
期刊：
影响因子：
2.6
通讯作者：
Hall, Ruth M.
中科院分区：
文献类型：
--
作者：
Ambrose, Stephanie J.;Harmer, Christopher J.;Hall, Ruth M.
在早期的一项研究中，根据相似性和强进入性，IncA和IncC质粒被归类为“A-C复合体”。然而，最近，IncA/C这个术语经常被用来描述属于这两个群体的质粒。假设原始报告中没有包含支持数据，并且一致的iteron序列已被证明基本上相同，我们再次解决了这个问题。将原始的IncA质粒RA1和IncC质粒pRMH760通过转化导入同一细胞，发现在没有选择质粒的情况下，它们可以稳定地维持100代以上，即它们是相容的。我们的结论是，使用术语IncA/C这一重要的质粒组确实是不正确的，它造成不必要的混淆。鉴于IncC质粒在抗生素耐药基因传播中的重要性，我们建议停止使用具有误导性的术语IncA/C、IncA/C-1和IncA/C-2。此外，RA1和pRMI-1760通过共轭转移完全阻止对方进入它们所在的细胞。
In an early study, IncA and IncC plasmids that were reported to be compatible were grouped as the "A-C complex" based on similarities and on strong entry exclusion. However, recently, the term IncA/C has been used frequently to describe plasmids belonging to both of these two groups. Granted that the supporting data was not included in the original reports and that the consensus iteron sequences have since been shown to be essentially identical, we have addressed the question again. The original IncA plasmid, RA1, and the IncC plasmid pRMH760, were introduced into the same cell by transformation, and were found to be maintained stably for over 100 generations in the absence of selection for either plasmid, i.e. they were compatible. We conclude that use of the term IncA/C for this important plasmid group is indeed incorrect and it causes unnecessary confusion. Granted the importance of IncC plasmids in the spread of antibiotic resistance genes, we recommend that use of the misleading terms IncA/C, IncA/C-1 and IncA/C-2 should cease. In addition, RA1 and pRMI-1760 were shown to each completely prevent entry of the other via conjugative transfer into the cell they reside in.